Browsing by Author "van Diepeningen, Anne D."
Now showing 1 - 2 of 2
- Results Per Page
- Sort Options
Item Biofilm formation and resistance to fungicides in clinically relevant members of the fungal genus fusarium(MDPI, 2018-01-16) Sav, Hafize; Rafati, Haleh; Öz, Yasemin; Dalyan-Cilo, Burcu; Mohammadi, Faezeh; İlkit, Macit; van Diepeningen, Anne D.; Seyedmousavi, Seyedmojtaba; Ener, Beyza; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0002-4803-8206; AAG-8523-2021; 15053025300Clinically relevant members of the fungal genus, Fusarium, exhibit an extraordinary genetic diversity and cause a wide spectrum of infections in both healthy individuals and immunocompromised patients. Generally, Fusarium species are intrinsically resistant to all systemic antifungals. We investigated whether the presence or absence of the ability to produce biofilms across and within Fusarium species complexes is linked to higher resistance against antifungals. A collection of 41 Fusarium strains, obtained from 38 patients with superficial and systemic infections, and three infected crops, were tested, including 25 species within the Fusarium fujikuroi species complex, 14 from the Fusarium solani species complex (FSSC), one Fusarium dimerum species complex, and one Fusarium oxysporum species complex isolate. Of all isolates tested, only seven strains from two species of FSSC, five F. petroliphilum and two F. keratoplasticum strains, recovered from blood, nail scrapings, and nasal biopsy samples, could produce biofilms under the tested conditions. In the liquid culture tested, sessile biofilm-forming Fusarium strains exhibited elevated minimum inhibitory concentrations (MICs) for amphotericin B, voriconazole, and posaconazole, compared to their planktonic counterparts, indicating that the ability to form biofilm may significantly increase resistance. Collectively, this suggests that once a surface adherent biofilm has been established, therapies designed to kill planktonic cells of Fusarium are ineffective.Item Fatal breakthrough infection with Fusarium andiyazi: New multi-resistant aetiological agent cross-reacting with Aspergillus galactomannan enzyme immunoassay(Wiley, 2014-04) van Diepeningen, Anne D.; Meijer, Martin; Al-Hatmi, Abdullah M.S.; Kebabçı, Nesrin; Ener, Beyza; Ersal, Tuba; Özkocaman, Vildan; Ursavaş, Ahmet; Çetinoğlu, Ezgi Demirdöğen; Akalın, Halis; Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Göğüs Hastalıkları Anabilim Dalı.; 0000-0002-4803-8206; AAJ-4354-2021; AAI-3169-2021; AAH-1854-2021; AAG-8523-2021; AAU-8952-2020; 56060994000; 15053025300; 56061031700; 6603145040; 8329319900; 57189524206; 57207553671Disseminated infections caused by members of the Fusarium fujikuroi species complex (FFSC) occur regularly in immunocompromised patients. Here, we present the first human case caused by FFSC-member Fusarium andiyazi. Fever, respiratory symptoms and abnormal computerised tomography findings developed in a 65-year-old man with acute myelogenous leukaemia who was under posaconazole prophylaxis during his remission-induction chemotherapy. During the course of infection, two consecutive blood galactomannan values were found to be positive, and two blood cultures yielded strains resembling Fusarium species, according to morphological appearance. The aetiological agent proved to be F. andiyazi based on multilocus sequence typing. The sequencing of the internal transcribed spacer region did not resolve the closely related members of the FFSC, but additional data on partial sequence of transcription elongation factor 1 alpha subunit did. A detailed morphological study confirmed the identification of F. andiyazi, which had previously only been reported as a plant pathogen affecting various food crops.