Browsing by Author "Alver, Oktay"
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Publication Acanthamoeba keratitis and acanthamoeba conjunctivitis: A case report(Iranian Scientific Society Medical Entomology, 2020-04-01) Alver, Oktay; Baykara, Mehmet; Yuruk, Merve; Ülkü Tüzemen, Nazmiye; ALVER, OKTAY; BAYKARA, MEHMET; TÜZEMEN, NAZMİYE ÜLKÜ; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı; 0000-0002-5555-1649; AAA-5241-2021; ABI-7051-2020; CGE-2103-2022Acanthamoeba species are vision-threatening agents by causing cornea infections known as Acanthamoeba keratitis. A 5 year-old kid with the complaints of erythema, eyelid edema, inflammation, limitation of eye movements in the right eye, and having no history of wearing contact lenses or trauma, was diagnosed of Acanthamoeba conjunctivitis through laboratory examinations in the Ophthalmology clinic. The visual sharpness of the patient improved after the treatment. A 44 year-old female patient suffering from pain, stinging, irritation, and inability to see in the left eye with the history of wearing contact lenses or trauma was diagnosed of Acanthamoeba keratitis through laboratory examinations. The agent was isolated and identified as "A. castellam" in the Genotype "T2". Examination of the left eye on the 15th day of treatment indicated that all complaints disappeared except for the cataract originated visual loss. However, the first diagnosis of Acanthamoeba keratitis appeared in the literature on a case with no history of wearing contact lenses and trauma it is found to be attention grabbing. We think that Acanthamoeba should not be ignored among microbial agents that cause eye infection with or without trauma and contact lens usage history.Item Cerebral malaria treated with artemisinin in the intensive care unit: A case report(Iranian Scientific Society Medical Entomology, 2016) Çizmeci, Elif Ayşe; Kelebek Girgin, Nermin; Ceylan, İlkay; Tuncel, Tekin; Alver, Oktay; Akalın, Emin Halis; Uludağ Üniversitesi/Tıp Fakültesi/Anesteziyoloji ve Yoğun Bakım Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0003-3306-3107; AAU-8952-2020; AAA-5241-2021; 56805220900; 8638070300; 57200337923; 57188585902; 24070021900; 57207553671Malaria is a parasitic disease that is starting to be encountered in intensive care units (ICU) worldwide, owing to increasing globalisation. Severe malaria caused by Plasmodium falciparum, is characterised by cerebral malaria, acute renal failure, hypoglycaemia, severe anaemia, splenomegaly and alveolar oedema. We present the case of a 25-yr old male patient who presented to the Emergency Department of Uludag University in Bursa, Turkey in the winter of 2014 with complaints of fever for three days. His medical history revealed a 14-month stay in Tanzania. Staining of blood smears revealed characteristic gametocytes in accordance with P. falciparum infection. The day after admission, he had an epileptic seizure after which his Glasgow Coma Scale was 6, so he was intubated and transferred to the ICU. A computerized tomography scan revealed findings of cerebral oedema. Intravenous mannitol was administered for 6 days. Intravenous artemisinin was continued for 10 days. Due to refractory fevers, anti-malarial treatment was switched to quinine and doxycycline on the 14th day and on the 16th day the fevers ceased. This case emphasizes that cerebral malaria should be suspected in cases of seizures accompanying malaria, and treatment should be initiated in the ICU. Furthermore, resistance of P. falciparum to artemisinin should be in mind when a response to therapy is lacking.Item A clinical scoring system for diagnosis of ocular demodicosis(Int Scientific Information, 2017-12-10) Alver, Oktay; Kıvanç, Sertaç Argün; Budak, Berna Akova; Tüzemen, Nazmiye Ülkü; Ener, Beyza; Özmen, Ahmet Tuncer; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı.; 0000-0002-4803-8206; 0000-0003-0995-5260; 0000-0002-0932-6977; AAA-5241-2021; AAG-8523-2021; A-4290-2018; AAH-6518-2021; ABE-3033-2020; 24070021900; 47861204900; 55370489800; 56100399900; 15053025300; 6701399730Background: Demodex may cause chronic and refractory blepharitis with associated ocular surface problems, and its diagnosis and treatment can be quite challenging. In this study, our aim was to assess the efficacy of tea tree oil in Demodex treatment on caucasian patients in an industrialized region of Turkey, and to develop a systematic scoring system for extremely accurate diagnosis in the absence of advanced facilities. Material/Methods: Charts of 412 patients with blepharitis were reviewed. A group of 39 out of 412 cases were identified as chronic and treatment-refractory, and therefore were enrolled in this study. Eyelashes from each of the lower and upper eyelids of both eyes were evaluated at x40 and x100 magnification using light microscopy. Treatment was started with 4% tea tree oil eyelid gel and 10% eyelash shampoo. Symptoms and findings were scored according to the most common complaints. Results: The mean age of the patients was 54.1 +/- 15.4 years. Seventeen (43.5%) patients were male and 22 (56.5%) patients were female. In 30 out of the 39 patients (76.9%) D. folliculorum was detected. Symptoms disappeared in 25 patients. The mean score of patients who were Demodex-negative was 2.7 +/- 1.0, and the mean score of patients who were Demodex-positive was 3.8 +/- 1.6 (p=0.047). Ninety-four percent of those with a score of 4 and over were found to be Demodex-positive (p=0.025). Conclusions: Treatment with tea tree oil can be successful. If there is no facility to identify Demodex under light microscopy, we recommend starting treatment for patients who have scores of 4 and over using the scoring chart developed in this study.Publication Determination of diagnostic value of cELISA for the diagnosis of anaplasmosis in clinically suspected ruminants(Kafkas Üniversitesi, 2015-09-01) Selçuk, Özgür; Alver, Oktay; Çatık, Serkan; Aydın, Levent; Şenlik, Bayram; Selçuk, Özgür; ALVER, OKTAY; Çatık, Serkan; AYDIN, LEVENT; ŞENLİK, BAYRAM; Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0003-2964-2245; AAA-5241-2021; JLA-7878-2023; JLJ-9087-2023; HLG-4450-2023; GBN-0139-2022The aim of this study was to determine diagnostic value of cELISA in anaplasmosis in clinically suspected animals and to compare the cELISA results with the clinical examination results. For this purpose a total of 720 ruminants (457 cattle, 146 sheep, 117 goat) were examined in terms of clinical signs. Eighty-eight ruminants consisting of 61 cattle, 11 sheep and 16 goat which had the symptoms of anemia, fever, icterus, weakness, depression and lack of appetite were selected for the study. Blood samples were collected from the jugular vein of all clinically suspected animals and serum samples were separated. A commercially available competitive enzyme-linked immunosorbent assay (C-ELISA) kit was used for determine antibodies to Anaplasma species. cELISA based diagnosis revealed that 47 of 88 serum samples (53.4%) were positive for anaplasmosis. In serological examination Anaplasma specific antibodies were determined in 45.9% of cattle, 63.6% of sheep and 56.2% of goats. Seropositivity rate was statistically differ among the age groups of cattle and the highest seropositvity rate was found in <12 month age (P < 0.005). However no difference was found in the seropositivity rate of Anaplasma in sheep and goat in relation to age group. From the data obtained in this study it can be concluded that clinical findings are not sufficient criteria for the diagnosis of anaplasmosis and must be supported by serological examination.Publication Evaluation of serum indirect haemagglutination test results of suspected cystic echinococcosis cases from 2009-2017(Pakistan Medical Assoc, 2022-06-01) Tuzemen, Nazmiye Ulku; Alver, Oktay; Ozakin, Cuneyt; Ener, Beyza; Tuzemen, Nazmiye Ulku; TÜZEMEN, NAZMİYE ÜLKÜ; Alver, Oktay; ALVER, OKTAY; Ozakin, Cuneyt; ÖZAKIN, CÜNEYT; Ener, Beyza; ENER, BEYZA; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; A-4290-2018Objective: This study aims to evaluate the serological, radiological and epidemiological analysis of suspected cystic echinococcosis patients, and to assess the positivity rate in the region. Method: The retrospective study was conducted at Bursa Uludag University Hospital, Turkey and comprised data from January 2009 to December 2017 related to patients of either gender with suspected cystic echinococcosis who underwent indirect haemagglutination testing. Demographic and clinical data of patients who tested positive were analysed. Statistical analysis was done using SPSS 23. Results: Of the 3910 patients with a mean age of 41.6 +/- 19.35 years (range: 0-93 years) who underwent indirect haemagglutination testing, 692(17.7%) tested positive; 390(56.4%) females, and 302(43.6%) males. The highest seropositivity rate 107(15.5%) was observed in 2011, followed by 104(15%) in 2016. Seropositive cases were predominantly seen in those aged 40-49 years 131 (18.9%), followed by those aged 50-59 years 124 (17.9%). Conclusion: Cystic echinococcosis was found to be a public health problem in South Marmara region of Turkey.Item Facial abscess-like conglomerates associated with Demodex mites(Wiley, 2006-09) Aydoğdu, Kenan; Alver, Oktay; Töre, Okan; Karadoğan, S. Kenan; Uludağ Üniversitesi/Tıp Fakültesi/Dermatoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı/İmmünoloji Birimi.; 0000-0002-0193-1128; AAA-5241-2021; AAH-6216-2021; 9739755800; 24070021900; 6505909596; 9738885800Demodex folliculorum and Demodex brevis are normal inhabi-tants of the human pilosebaceous duct and sebaceousglands, respectively, with a predilection to colonize facialskin. 1 Parasitism is usually asymptomatic, but Demodex mites have been implicated as a pathogenic agent for somesuppurative or granulomatous dermatological conditionsresembling suppurative folliculitis of face and scalp inimmunocompetent and immunocompromised patients. Demodex infestation is usually associated with pruritus,erythema, papules, pustules, nodules or scales with apredilection for the face, especially the nose and nasolabialfolds in an immunocompetent patient.2–6 We describe herethe case of a 40-year-old man with acute onset papules,pustules and abscess-like conglomerates associated withheavy infestation of Demodex mites on his face.Item Genç yaştaki kolorektal kanser tanılı hastada blastocytis hominis enfeksiyonunun klinik önemi: Olgu sunumu(Uludağ Üniversitesi, 2006-08-09) Şenol, Emel; Kıyıcı, Murat; Alver, Oktay; Tolunay, Şahsine; Uludağ Üniversitesi/Tıp Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Gastroenteroloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.Kolorektal kanser ileri yaş hastalığı olarak bilinmektedir. Son yıllarda genç hastalarda kolorektal kanser sıklığında artış ve yaşam beklentisinin de kötü olduğu bildirilmektedir. Blastocystis hominis kolonun normal florasında varlığı saptanmış ancak klinik önemi tartışılmakta olan bir protozoondur. Burada Blastocystis hominis enfeksiyonunun eşlik ettiği kolorektal taşlı yüzük hücreli kanser tanısı alan genç bir olgu literatür eşliğinde tartışıldı.Item HIV/AIDS hastalarında periferik kan örneklerinde real-time PCR’ın toksoplazmoz tanısındaki yerinin değerlendirilmesi(Bursa Uludağ Üniversitesi, 2023-02-20) Kır, Goncagül; Alver, Oktay; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Tp Fakültesi/Tıp-Mikrobiyoloji Anabilim Dalı.; 0000-0003-1055-1108Zorunlu hücre içi bir protozoon olan Toxoplasma gondii’nin neden olduğu toksoplazmoz, immünsuprese kişilerde önemli morbidite ve mortalite sebeplerindendir. İmmünkompetan kişilerde toksoplazmoz tanısında kullanılan serolojik testler, HIV’le yaşayan (Human Immunodeficiency Virus/ İnsan İmmün Yetmezlik Virüsü) bireylerde antikor yanıtı olmadığından güvenilir değildir ve moleküler testler ile doğrulanmalıdır. Çalışmamızda HIV’li hasta grubunda, düşük antikor yanıtı nedeniyle oluşabilecek yanlış negatiflikleri qPCR (Gerçek Zamanlı Polimeraz Zincir Reaksiyonu) testi ile doğrulayarak, toksoplazmoz koenfeksiyonunun teşhisinde moleküler tabanlı testin uygulanabilirliğinin belirlenmesi amaçlanmıştır. Bursa Uludağ Üniversitesi Enfeksiyon Hastalıkları polikliniğine randevulu olarak gelen HIV pozitif, Anti-Toxoplasma IgG ve Anti-Toxoplasma IgM (BioMérieux, France) istemi yapılan toplam 137 gönüllü çalışmaya dahil edilmiştir. Çalışmaya dahil edilen gönüllülerden EDTA’lı tüpe kan alınarak, buffy coat ayrıştırılmış ve DNA Mini Kit (Qiagen, Almanya) kullanılarak ekstraksiyon yapılmıştır. QuantiNova Probe PCR Kit (QiaGen, Germany) ve Primer Design Advenced Kit (Genesig, UK) kullanılarak amplifikasyon gerçekleştirilmiştir. PCR testi sonuçları Anti-Toxoplasma IgG ve Anti-Toxoplasma IgM, CD4+T lenfosit sayısı, hastaların kullandığı tedaviler ve demografik özellikleri ile karşılaştırılmıştır. Gerçek Zamanlı PCR ile çalışılan 137 örnekten, iki örnekte (%1,45) T. gondii DNA’sı saptanmıştır ve bu iki hasta toksoplazmoz açısından seronegatif olarak bulunmuştur. Bu çalışmada pozitif çıkan iki hastanın birinde antiretroviral tedavinin yanı sıra profilaktik tedavi kullanmasına rağmen, T. gondii DNA’sı saptanmış olması ve iki hastanın da toksoplazmoz açısından seronegatif olması moleküler testlerin önemini vurgulamaktadır.Publication Investigation of parasitic infection rate in stool samples submitted to uludag university parasitology laboratory between 2011-2015(Bilimsel Tip Yayinevi, 2017-01-01) Tüzemen, Nazmiye Ülkü; TÜZEMEN, NAZMİYE ÜLKÜ; Alver, Oktay; Ener, Beyza; ENER, BEYZA; ALVER, OKTAY; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı.; 0000-0003-3544-3509; AAA-5241-2021; A-4290-2018; AAG-8523-2021Introduction: Intestinal parasitic infections are among the most significant causes of morbidity and mortality in undeveloped countries, particularly in children. These infections may cause loss in physical and mental progress of children in particular, and loss of work and labour force in adults.Materials and Methods: In this study, patients who applied with various gastrointestinal complaints to the clinics of the Uludag University Medical Faculty, were thoroughly investigated for the presence of intestinal parasites. A total of 8981 stool and 854 cellophane tape samples were parasitologically evaluated. All stool samples were prepared using formal-ethyl acetate concentration method for helminth ova and protozoan cysts, and examined in lugol preparations microscopically with 10x and 40x magnifications. Preparations were examined by using oil-immersion objectives (100x) following trichrome and modified Erlich-Ziehl-Nielsen staining for the diagnosis of intestinal and coccidian protozoa, respectively. For the detection of Entamoeba histolytica adezin antigen in stools, commercial ELISA kit (Wampole (R) E. histolytica II Test Kit; TechLab, USA) was used.Results: In this study, one or more parasites were found in 327 (3.6%) of the 8981 stool samples (including nonpathogenic protozoa). Enterobius vermicularis eggs were detected in 29 (3.4%) out of 854 samples by using the cellophane tape method. Of the parasite detected cases, 165 (50.5%) were female and 162 (49.5%) were male. Giardia intestinalis (0.9%) and E. vermicularis (3.4%) were the most frequently detected protozoon and helmint parasites, respectively. The parasites were detected mostly in summer months (26.3%).Conclusion: Although the prevalence rates of intestinal parasites were lower than those in the previous studies carried out in the city, it is seen that the presence of intestinal parasites is still a serious public health problem in our region.Item Nocardia cyriacigeorgica: Pulmonary infection in a patient with Basedow-Graves disease and a short review of reported cases(Elsevier, 2008-05) Akçaĝlar, Sevim; Ersoy, Canan; Yılmaz, Emel; Heper, Yasemin; Alver, Oktay; Akalın, Halis; Ener, Beyza; İmamoğlu, Şazi; Töre, Okan; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0002-4803-8206; AAG-8523-2021; AAU-8952-2020; AAH-6506-2021; AAA-5241-2021; AAH-8861-2021; 6506194958; 6701485882; 22037135100; 56191003300; 24070021900; 57207553671; 15053025300; 6602297533; 6505909596Item Nosocomial diarrhea and Clostridium Difficile associated diarrhea in a Turkish University Hospital(Elsevier France, 2009-06) Bakker, Dennis; Kuijper, Ed J.; Ergen, E. K.; Akalın, Halis; Yılmaz, Emel; Sınırtaş, Melda; Alver, Oktay; Heper, Yasemin; Özakın, Cüneyt; Ener, Beyza; Mstık, Reşit; Helvacı, Safiye; Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Mikrobiyoloji Anabilim Dalı.; 0000-0002-3894-1231; 0000-0002-4803-8206; AAH-6506-2021; AAG-8523-2021; AAA-5241-2021; AAU-8952-2020; 35078341200; 57207553671; 22037135100; 6505818048; 24070021900; 56191003300; 57200678942; 15053025300; 6602564624; 6602103491Background. - Clostridium difficile (C. difficile) is a well-established cause of nosocomial diarrhea. The aim of our study was to define the incidence of nosocomial diarrhea in our hospital and to determine the role of C. difficile. Additionally, the risk factors for nosocomial diarrhea and Clostridium difficile associated diarrhea (CDAD) were investigated. Methods. - We included all patients, 18 years of age or more, who were admitted to the Uludag Teaching Hospital between October 1, 2004 and February 1, 2005, and developed diarrhea at least three days after hospital admission. A case-control study was per-formed. Results. - The total incidence of nosocomial diarrhea was 0.6 per 1,000 hospitalization-days and 5 per 1,000 patients' admissions. Previous use of chemotherapy was found to be an important predisposing factor for nosocomial diarrhea. The incidence of CDAD was 0.26 per 1,000 hospitalization-days and 2.1 per 1,000 admissions, comparable with incidence rates in Europe. CDAD was diagnosed in 43% of patients with nosocomial diarrhea. No severe cases of CDAD were diagnosed. A correlation was found between CDAD and antibiotic use before admission and during admission in univariate analysis. PCR ribotyping revealed four strains of PCR ribotype 002 and I strain of ribotype 0 12 Out of 5 C difficile strains available for extensive identification. Conclusion. - The incidence rates of nosocomial diarrhea and CDAD are not different than the usual incidence rates in Europe. C. difficile was the causative agent in 43% of patients with nosocomial diarrhea.Item Prevalence of intestinal parasites in Bursa Province of Turkey and assessment of enzyme-linked immunosorbent assays (ELISA) and three microscopic methods in the diagnosis of Entamoeba histolytica/Entamoeba dispar(Academic Journals, 2011-06) Alver, Oktay; Heper, Yasemin; Ercan, İlker; Akalın, Halis; Töre, Okan; Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0002-2382-290X; AAH-6506-2021; AAA-5241-2021; AAU-8952-2020This study was designed to determine the prevalence of intestinal parasites in relation to personal and environmental risk factors in Bursa province of Turkey and to compare wet mount + Lugol's iodine, formalin ethyl acetate concentration, trichrome staining and enzyme-linked immunosorbent assays (ELISA) methods used in Entamoeba histolytica/Entamoeba dispar diagnosis. For this purpose a total of 176 faecal samples from people were randomly collected from regions of the Bursa City Centre, where crowded families of low socio-economic levels reside. All faecal samples were examined by wet mount + Lugol's iodine formalin ethyl acetate concentration, trichrome staining methods and ELISA. 66 faecal samples were infected with one or more parasite species and the overall prevalence rate was 37.5%. Nine species of intestinal parasites, including six protozoon and three helminth species were found. The most prevalent species was Blastocystis hominis (17.04%) and others were found with the following frequencies: 9.09% Enterobius vermicularis, 7.38% Giardia intestinalis, 5.68% Entamoeba coli, 2.27% E. histolytica/E. dispar, 1.13 % Entamoeba hartmani, 1.13% Taenia spp., 0.56% Hymenolepis nana. 6.2% of examined samples had mixed infections. Overall prevalence of the detected parasites did not differ significantly in different demographic groups. Except for B. hominis and G. intestinalis, there was no evidence for sex, age and other demographic-related differences in the prevalence of these parasites. Statistically, B. hominis and G. intestinalis were higher in 20 to 49 and in 1 to 19 year age groups, respectively than in any other age group. Microscopical examination and ELISA revealed that one sample (0.5%) was found to be positive using all 4 methods, and 162 samples (92%) were found to be negative with all 4 methods applied. E. histolytica/E. dispar complex was detected in 0.56% (1); in 0.56% (1), in 0.56% (1) and in 2.27% (4) of the fecal samples examined by wet mount + Lugol's iodine, formalin ethyl acetate concentration, with trichrome staining, and ELISA respectively. The wet mount + Lugol's iodine, formalin ethyl acetate and trichrome staining methods had a sensitivity of 25% each, a specificity of 94.1, 99.4 and 98.2%, compared to the results of the E. histolytica/E. dispar ELISA, respectively.Item The role of Demodex folliculorum in chronic, treatment resistant blepharitis and clinical outcome(Assoc Research Vision Ophthalmology, 2017-06) Budak, Berna Akova; Kıvanç, Sertaç Argün; Alver, Oktay; Yücel, Ahmet Ali; Ener, Beyza; Tüzemen, Ülkü; Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı.; 0000-0003-0995-5260; 0000-0002-4803-8206; AAA-5241-2021; AAH-6518-2021; ABE-3033-2020; AAG-8523-2021