Turgut, T.Lopez, Maria CeciliaUemura, HiroshiBaker, Henry V.2022-04-202022-04-202003-03Türkel, S. vd. (2003). “Mutations in GCR1 affect SUC2 gene expression in Saccharomyces cerevisiae”. Molecular Genetics and Genomics, 268(6), 825-831.1617-4615https://doi.org/10.1007/s00438-003-0808-4https://link.springer.com/article/10.1007/s00438-003-0808-4http://hdl.handle.net/11452/25893Transcription of SUC2, the gene that encodes the cytoplasmic and secreted forms of the enzyme invertase, is controlled by glucose repression and derepression mechanisms in Saccharomyces cerevisiae. Several regulatory factors such as the Mig1p-Tup1p-Ssn6p repressor complex and the Snf1p kinase complex have been identified previously as regulators of SUC2 expression. We show that, in addition to these factors, expression of SUC2 is affected by mutations in the gene GCR1 that encodes the glycolysis regulatory protein Gcr1p. Expression of Suc2-LacZ was not repressed by glucose in gcr1 mutant yeast cells exposed to glucose. Furthermore, secreted invertase activity was constitutively expressed under glucose-repressed and derepressed conditions in gcr1 mutants. DNA gel mobility shift assays and in-vitro DNase I protection experiments mapped a DNA binding site for Ger1p in the transcriptional control region of the SUC2 gene, next to a previously mapped Mig1p binding site. However, the mechanism by which gcr1 mutations relieve glucose repression remains obscure.eninfo:eu-repo/semantics/closedAccessBiochemistry and molecular biologyGenetics and heredityEukaryotaSaccharomyces cerevisiaeSUC2InvertaseGCR1Glucose repressionRepressor-activator protein-1Glucose repressionTranscriptional activationChromatin-structureMessenger-RNASYeastPromoterBindingSequenceSNFArticle0001824566000142-s2.0-0345700687825831268612655409Biochemistry and molecular biologyGenetics and heredityRibosomes; Budding Yeast; Promoter RegionArticleBinding siteCell mutantEnzyme activityEnzyme releaseGene expression regulationGene mutationNonhumanPriority journalProtein DNA bindingSaccharomyces cerevisiaeTranscription regulationYeast cellBeta fructofuranosidaseBeta galactosidaseDNA binding proteinFungal DNAFungal proteinGlucoseGlycolysis regulatory protein 1Hybrid proteinIsoenzymeMutant proteinProtein Mig1Protein SUC2Unclassified drug