Uzun, LokmanDenizli, Adil2022-10-042022-10-042013-10-01Osman, B. vd. (2013). "Microcontact imprinted surface plasmon resonance sensor for myoglobin detection". Materials Science and Engineering C-Metarials for Biological Applications, 33(7), 3609-3614.0928-49311873-0191https://doi.org/10.1016/j.msec.2013.04.041https://pubmed.ncbi.nlm.nih.gov/23910256/http://hdl.handle.net/11452/28953In this study, we prepared surface plasmon resonance (SPR) sensor using the molecular imprinting technique for myoglobin detection in human serum. For this purpose, we synthesized myoglobin imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-t-tryptophan methyl ester) [poly(HEMA-MATrp)] nanofilm on the surface of SPR sensor. We also synthesized non-imprinted poly(HEMA-MATrp) nanofilm without myoglobin for the control experiments. The SPR sensor was characterized with contact angle measurements, atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry. We investigated the effectiveness of the sensor using the SPR system. We evaluated the ability of SPR sensor to sense myoglobin with myoglobin solutions (pH 7.4, phosphate buffer) in different concentration range and in the serum taken from a patient with acute myocardial infarction. We found that the Langmuir adsorption model was the most suitable for the sensor system. The detection limit was 87.6 ng/mL. In order to show the selectivity of the SPR sensor, we investigated the competitive detection of myoglobin, lysozyme, cytochrome c and bovine serum albumin. The results showed that the SPR sensor has high selectivity and sensitivity for myoglobin.eninfo:eu-repo/semantics/closedAccessMaterials scienceMolecularly imprinted polymerMicrocontact imprintingSPR sensorMyoglobinNanofilmMyocardial-infarctionProtein recognitionRibonuclease-aPolymersLysozymeNanoparticlesCompositeBiosensorDiagnosisCryogelsAmino acidsAtomic force microscopyBody fluidsPhotoelectronsPlasmonsSensorsX ray photoelectron spectroscopyMicrocontact imprintingMolecularly imprinted polymerMyoglobinNanofilmSPR sensorMicro-contact imprintingMolecularly imprinted polymerMyoglobinNano filmsSPR sensorsSurface plasmon resonanceAnimalsCattleComputer systemsHumansKineticsMolecular imprintingMyoglobinRecyclingSerum albumin, bovineSurface plasmon resonanceSurface propertiesTemperatureArticle0003254457000062-s2.0-848811731623609361433723910256Materials science, biomaterialsMagnetism; 4-Vinylpyridine; Solid Phase ExtractionBovine serum albuminMolecularly imprinted polymerMyoglobinNanofilmMyoglobinAnimalArticleCattleComputer systemEquipmentHumanKineticsMethodologyMicrocontact imprintingMolecular imprintingRecyclingSPR sensorSurface plasmon resonanceSurface propertyTemperatureBovinaeDevicesMolecular imprintingProceduresSurface plasmon resonance