Aher, GeoffreyGinsburg, MikeMaassarani, GhanimaKotze, DirkKeskintepe, LeventKeskintepe, MeralBaltacı, VolkanÜnsal, Evrim2022-08-162022-08-162007-05Sher, G. vd. (2007). "Oocyte karyotyping by comparative genomic hybrydization provides a highly reliable method for selecting "competent" embryos, markedly improving in vitro fertilization outcome: A multiphase study". Fertility and Sterility, 87(5),1033-1040.00150282https://doi.org/10.1016/j.fertnstert.2006.08.108https://www.sciencedirect.com/science/article/abs/pii/S0015028206044475http://hdl.handle.net/11452/28204Objective: To assess the karyotypic relationship between prefertilized/postfertilized oocytes and embryos using comparative genomic hybridization (CGH) on polar body-1 (PB-1), PB2, and blastomere biopsies and to evaluate IVF outcomes after transfer of blastocysts derived from euploid oocytes. Design: Prospective cohort. Setting: Medical center. Patient(s): Phase1: Fourteen oocyte donors (23-29 years). Phase 2: Forty-one healthy embryo recipients aged 29-43 years free of endometrial implantation dysfunction. In 30 cases own eggs were used. Eleven women used donated oocytes. Intervention(s): Phase 1: PB-1 biopsies followed intracytoplasmic sperm injection (ICSI), PB-2, and day 3 blastomere biopsies. Phase 2: PB-1 biopsy followed by ICSI using normal sperm and the subsequent embryo transfer of ≤2 blastocysts derived from euploid oocytes. Comparative genomic hybridization on all DNA derived from phase 1 and 2 biopsies. Main Outcome Measure(s): Pregnancy and implantation rate. Result(s): Phase 1: 39% of oocytes and 88% of zygotes were euploid; >95% progressed to blastocysts. Mosaicism as evidenced by euploid oocytes developing into aneuploid zygotes or embryos occurred in 13% of concepti. Phase 2: Six of 30 women using own eggs, who failed to produce euploid oocytes, were cancelled. Thirty-five women underwent embryo transfers with ≤2 (mean, 1.3 ± 0.7) blastocysts derived from euploid oocytes. The ongoing pregnancy/implantation rates per embryo transfer were 74% and 82%, respectively. Conclusion(s): Transferring euploid embryos markedly improved IVF outcome. These findings, if corroborated, could initiate a paradigm shift in assisted reproductive technology (ART).eninfo:eu-repo/semantics/closedAccessComparative genomic hybridizationEmbryo transferImplantationKaryotypePregnancyPreimplantation genetic diagnosisSitu hybridization analysisChromosome-abnormalitiesImplantation failureAneuploidySusceptibilityRecombinationMorphologyWomenIvfCohort studiesAdultFemaleNucleic acid hybridizationFertilization in vitroProspective studiesGenome, humanHumansKaryotypingOocytesPregnancyPregnancy rateArticle0002465836000072-s2.0-342475173401033104087517258713Obstetrics & gynecologyReproductive biologyPreimplantation Genetic Diagnosis; Aneuploidy; BlastocystAdultIntracytoplasmic sperm injectionCohort analysisControlled clinical trialClinical articleControlled studyDNA hybridizationFertilization in vitroArticleOutcome assessmentBiopsyFetus karyotypingBlastomereClinical trialFemaleHumanNidationOocytePregnancyPriority journalZygote