Sağ, Şebnem ÖzemriYakut, TahsinGörükmez, ÖzlemGörükmez, OrhanTüre, MehmetKarkucak, MutluGülten, TunaAli, Rıdvan2024-08-082024-08-082015-10-011945-0265https://doi.org/10.1089/gtmb.2015.0056https://www.liebertpub.com/doi/10.1089/gtmb.2015.0056https://hdl.handle.net/11452/43793Aims: Fluorescence in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (QRT-PCR) were used to diagnose or screen for minimal residual disease (MRD) in Philadelphia (Ph) chromosome-positive leukemia. We compared the diagnostic utility of FISH and QRT-PCR at various time points in the course of chronic myelogenous leukemia (CML) and to determine the mean initial values for patients whose QRT-PCR results were not known at the time of diagnosis. Results: We analyzed 135 results for 78 CML patients tested by FISH and QRT-PCR for the Ph chromosomal translocation. All newly diagnosed cases were positive by both methods. On follow-up following treatment, 1 case was FISH positive and QRT-PCR negative; 61 cases were FISH negative and QRT-PCR positive. Overall concordance was 54.1%. There was good concordance between QRT-PCR results and cytogenetic response categories. Conclusions: We confirmed that QRT-PCR allows precise measurement of low levels of BCR-ABL transcripts and can serve as a sensitive indicator of MRD. We also demonstrated 100% correlation between QRT-PCR and FISH in newly diagnosed CML.eninfo:eu-repo/semantics/closedAccessChronic myeloid-leukemiaPolymerase-chain-reactionRt-PCRFollow-upPositive leukemiasPeripheral-bloodInterferon-alphaResidual diseaseFishCytogeneticsBiochemistry & molecular biologyGenetics & heredityArticle000362269300009584588191010.1089/gtmb.2015.0056