Ardıçlı, ÖzgeDemirbilek, Serpil KahyaCarlı, Kamil Tayfun2024-10-022024-10-022022-01-010025-8628https://doi.org/10.21521/mw.6606http://www.medycynawet.edu.pl/archives/423/6606-summary-med-weter-78-01-6606-2022https://hdl.handle.net/11452/45645This study aimed to isolate aerobic and microaerophilic bacteria from mastitis milk samples, as well as to determine their antibiotic resistance. A total of 196 bovine mastitis milk samples were tested by standard bacteriological methods and with API identification test kits. Antimicrobial susceptibility testing was performed by the Kirby-Bauer disk diffusion method. The results revealed that the predominant isolate was S. aureus, with an isolation rate of 28%, followed by Streptococcus spp. (27%) and E. coli (19%). Isolation rates for Corynebacterium spp., Mycoplasma spp., and Pseudomonas aeruginosa were 11%, 6%, and 4%, respectively. Compared to the bacteria mentioned above, lower percentages were observed for Trueperella pyogenes (2%), Pasteurella multocida (2%), and Klebsiella pneumoniae (1%). A broad evaluation of antimicrobial resistance showed that the pathogens were resistant to tetracycline (68.63%), oxytetracycline (41.57%), ampicillin (39.08%), ceftiofur (38.1%), cephalexin (32.26%), penicillin (31.25%), amoxicillin/clavulanic acid (24.53%), enrofloxacin (24.44%), gentamycin (23.68%), and trimethoprim/sulfamethoxazole (22.09%). This study demonstrated that the sources of bacteria isolated from mastitis bovine milk samples were both contagious and environmental. More importantly, the present results demonstrate a critically high antimicrobial resistance in dairy cattle. For instance, E. coli isolates showed a crucial resistance to commonly used and recommended antimicrobials, including ceftiofur (100%), cephalexin (83.33%), and tetracycline (94.44%). The results of this study may provide valuable information about clinical aspects of bovine mastitis infections and current antimicrobial resistance levels in dairy cattle.eninfo:eu-repo/semantics/openAccessSubclinical mastitisDairy-cowsStaphylococcus-aureusStreptococcus-dysgalactiaeEtiologic agentsUdder pathogensSusceptibilityPrevalenceHerdsLactationMastitisAntimicrobial resistanceDairy cattleBacterial strainsScience & technologyLife sciences & biomedicineVeterinary sciencesArticle000738136200003192478110.21521/mw.6606