Mulet, MagdalenaSatıcıoğlu, İzzet BurçinGomila, MargaritaLalucat, JorgeGarcia, Elena Valdes2023-01-092023-01-092020-06-12Duman, M. vd. (2020) ."Pseudomonas sivasensis sp. nov. isolated from farm fisheries in Turkey". Systematic and Applied Microbiology, 43(4).0723-20201618-0984https://doi.org/10.1016/j.syapm.2020.126103https://www.sciencedirect.com/science/article/pii/S0723202020300588http://hdl.handle.net/11452/30320A study of 91 isolates from fish farms in Turkey showed that isolates P7(T), P11, P24b, P29, P72, P73 and P158 belonged to the genus Pseudomonas according to 16S rRNA nucleotide sequence analysis. The analysis of the sequences of the RNA polymerase sigma factor gene (rpoD) located these strains in the Pseudomonas fluorescens lineage of species within the P. fluorescens subgroup, close to the cluster composed of the species Pseudomonas grimontii, Pseudomonas marginalia and Pseudomonas panacis. Based on similarities in the 16S rRNA and rpoD gene sequences of three previously isolated strains from other origins (CCUG 57209, CCUG 62357 and W5.2-93) linked them to the same cluster. A polyphasic taxonomic approach including phenotypic characterization, fatty acid composition, and multilocus sequence analysis, together with whole-cell MALDI-TOF data, corroborated this assumption. The genome G+C mol% contents were 59.48 and 59.71, respectively. The average nucleotide indices based on BLAST analysis and the genome-to-genome distance calculation for the P7T and CCUG 57209 strains with their closest relative, P. grimontii, were 88.16-88.29% and 38.10-38.20%, respectively. These data confirm that isolates P7(T), P11, P24b, P29, P72, P73, P158, CCUG 57209, CCUG 62357 and W5.2-93 represent a new species for which the name Pseudomonas sivasensis is proposed, with P7(T) as a type strain (=CCUG 74260(T)= and CECT30107(T)).eninfo:eu-repo/semantics/closedAccessBiotechnology & applied microbiologyMicrobiologyPseudomonasAquacultureWaterRainbow troutOnchorhynchus mykissWaterAeruginosaBacteriaProposalTreesAnimalsDNA, bacterialFatty acidsFisheriesGenes, bacterialGenome, bacterialNucleic acid hybridizationOncorhynchus mykissPhenotypePhylogenyPseudomonasRNA, ribosomal, 16SSequence analysis, DNATurkeyWater microbiologyArticle0005501236000022-s2.0-8508703314843432690194Biotechnology & applied microbiologyMicrobiologyGenome; Multilocus Sequence Typing; Nucleic Acid HybridizationFatty acidRNA 16SWaterBacterial DNAFatty acidRNA 16SArticleBacterial geneBacterial strainBacterium isolationDNA sequencingFatty acid analysisFisheryGene amplificationGene sequenceGenetic distanceLipid compositionMatrix assisted laser desorption ionization time of flight mass spectrometryMultilocus sequence typingNew speciesNonhumanNucleotide sequenceOncorhynchus mykissPhenotypePhylogenetic treePhylogenyPolymerase chain reactionPriority journalPseudomonasPseudomonas fluorescensPseudomonas grimontiiPseudomonas marginalisPseudomonas panacisPseudomonas sivasensisRNA polymerase sigma factor geneSpecies differentiationTurkey (republic)AnimalBacterial genomeChemistryClassificationCytologyDNA sequenceFisheryGeneticsMicrobiologyNucleic acid hybridizationOncorhynchus mykissPhysiologyPseudomonasTurkey (bird)