Gökçe, Elif2024-05-152024-05-152019-05Alçay, S. vd. (2019). "Long term incubation resilience of post-thaw ram semen diluted with lecithin-based extender supplemented with bovine serum albumin". Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25(3), 291-297.1300-6045https://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2461.pdfhttps://hdl.handle.net/11452/41439The objective of the study was to determine the optimum concentration of BSA in lecithin-based extender for post-thawing quality and incubation resilience (0 h, 6 h and 10 h) of ram sperm. Ejaculates were collected from five rams via electro ejaculation. Ejaculates were mixed to obtain pooled semen.Then, pooled semen was diluted with soybean lecithin-based extender without BSA (control) or supplemented with different concentrations of BSA (2.5 mg/mL, 5 mg/mL, 7.5 mg/mL and 10 mg/mL), at a final concentration of 150x106 spermatozoon/mL. Sperm motility, plasma membrane functional integrity (HOST), mitochondrial activity (rhodamine123), capacitation status (CTC), and DNA integrity (TUNEL) were evaluated. At the 10 h incubation, motility, plasma membrane functional integrity and mitochondria! function were better preserved in the BSA5 group compared to the control group. It was determined that high doses of BSA (5 mg/mL, 7.5 mg/mL and 10 mg/mL) affected acrosome reaction.The highest acrosome reaction rates were obtained in BSA10 groups in 6 h and 10 h incubation (P<0.05). TUNEL assay demonstrated that there were no differences among groups for DNA fragmentation at post-thaw and during incubation periods. The study shows that BSA supplemented extenders may have beneficial effect on ram semen parameters at 0 h, 6 h and 10 h of incubation. The results of the present study demonstrated a remarkable advantage of using 5 mg/mL of BSA in 1% lecithin-based extender.eninfo:eu-repo/semantics/openAccessBovine serum albuminCryopreservationIncubation resilienceRam spermatozoaSoybean lecithinEgg-yolkSperm capacıtatıonDNA fragmentationLiquid storageQualityAntioxidantsSpermatozoaMotilityCryopreservationVeterinary sciencesClinical & life sciencesReproductive biologySpermatozoaGood health and well-beingArticle0004605031000022-s2.0-85067432770291297253https://doi.org/10.9775/kvfd.2018.20843Veterinary sciencesExtender; Semen; Semen ExtendersBovine serum albuminPhosphatidylcholineAcrosome reactionArticleDNA fragmentationEjaculationFreeze thawingIncubation resilienceMedical parametersMetabolic activity assayNonhumanPhysical phenomenaPlasma membrane functional integrityPost thaw semenRam (sheep)SpermSperm qualitySpermatozoon capacitation