Zorluoglu, Abdullah2022-11-112022-11-112013-04Tunca, B. vd. (2013). "Overexpression of CK20, MAP3K8 and EIF5A correlates with poor prognosis in early-onset colorectal cancer patients". Journal of Cancer Research and Clinical Oncology, 139(4), 691-702.0171-52161432-1335https://doi.org/10.1007/s00432-013-1372-xhttps://link.springer.com/article/10.1007/s00432-013-1372-xhttp://hdl.handle.net/11452/29456Due to ethnic, genetic and environmental factors, the clinical and molecular characteristics of Turkish colorectal cancer (CRC) patients are different from those of Western populations. The aim of this study was to clarify the relevant alterations of gene expression associated with colorectal carcinogenesis in early-onset patients and to identify specific biomarkers that could provide novel therapeutic molecular targets in this population. The expression profiles of 114 different genes were evaluated using mRNA PCR arrays in 39 tumors and 20 surgical margin tissue samples from 39 sporadic CRC patients diagnosed at less than 50 years of age. The expression levels of IMPDH2, CK20, MAP3K8 and EIF5A were strongly up-regulated in CRC tissues compared with normal colorectal tissues (p < 0.05). The highly significant expression ratios of CK20, MAP3K8 and EIF5A observed in the colorectal tumors of patients predicted recurrence (p < 0.05). The expression of IMPDH2, CK20, MAP3K8 and EIF5A was significantly higher in the tumors of patients with short median survival (log-rank p value < 0.05). Progression-free survival was also significantly increased in patients with low expression of the EIF5A gene compared with those who exhibited high expression of this gene (log-rank p value < 0.05). We demonstrated that high CK20, MAP3K8 and EIF5A expression levels were significant prognostic factors for poor overall survival in CRC patients. Further studies and validations are required; these genes may provide novel therapeutic molecular targets for CRC treatment, as well as new directions for the development of anticancer drugs.eninfo:eu-repo/semantics/closedAccessOncologyColorectal cancerCK20MAP3K8EIF5APoor prognosisTurkish populationInosine monophosphate dehydrogenaseGenomic hybridization revealsSquamous-cell carcinomaIndependent predictorExpressionCytokeratin-20Eif-5a2YoungGeneAgeAdolescentAdultCase-control studiesColonColorectal neoplasmsFemaleFollow-up studiesGene expression profilingHumansImmunoenzyme techniquesKeratin-20MaleMAP kinase kinase kinasesMiddle agedNeoplasm metastasisNeoplasm recurrence, localNeoplasm stagingOligonucleotide array sequence analysisPeptide initiation factorsPrognosisProto-oncogene proteins c-aktReal-time polymerase chain reactionRectumReverse transcriptase polymerase chain reactionRNA, messengerRNA-binding proteinsSurvival rateTumor markers, biologicalYoung adultArticle0003168177000182-s2.0-84876414952691702139423322277OncologyFactor EF-P; RNA-Binding Protein; Eukaryotic Translation Initiation Factor 5AInitiation factor 5AInosinate dehydrogenaseMitogen activated protein kinase kinase kinaseMitogen activated protein kinase kinase kinase 8Unclassified drugAdultArticleCancer prognosisCancer recurrenceCancer stagingCK20 geneClinical articleColon carcinogenesisColorectal cancerDisease free survivalDNA determinationEarly cancerEukaryotic translation initiation factor 5A geneFemaleGeneGene expression profilingGene expression regulationGene overexpressionHumanHuman tissueImmunohistochemistryIMP inosine monophosphate dehydrogenase geneMaleMitogen activated protein kinase kinase kinase 8 geneNucleotide sequenceOverall survivalPolymerase chain reactionPriority journalProgression free survivalSurvival timeUnindexed sequence