2022-01-192022-01-192009Akesen, B. vd. (2009). "Evaluation of intraarticular collagenase, TIMP-1, and TNF-α levels before and after anterior cruciate ligament reconstruction". Acta Orthopaedica et Traumatologica Turcica, 43(3), 214-218.1017-995Xhttps://doi.org/10.3944/AOTT.2009.214https://www.aott.org.tr/en/evaluation-of-intraarticular-collagenase-timp1-and-tnf-levels-before-and-after-anterior-cruciate-ligament-reconstruction-134533http://hdl.handle.net/11452/24151Objectives: We investigated intra-articular levels of collagenase, which presumably promotes bone-tendon healing, and collagenase mediators involved in its production (tumor necrosis factor-alpha, TNF-alpha) and inhibition (TIMP-1 enzyme) in patients following anterior cruciate ligament (ACL) reconstruction. Methods: The study included 16 patients (15 males, 1 female; mean age 27 years; range 17 to 40 years) who underwent arthrocentesis due to effusion that developed following reconstruction of isolated ACL injuries. Intra-articular levels of collagenase, TNF-alpha, and TIMP-1 were measured using appropriate activity assay and immunoassay kits in synovial fluid samples obtained intraoperatively and during arthrocentesis. The mean time from ACL injury to surgical repair was 21 +/- 10 months (range 1 to 72 months). Arthrocentesis was performed in a mean of 18 hours (range 12 to 36 hours) following ACL repair. Results: Measurements in synovial fluid samples obtained intra- and postoperatively yielded 1.49 +/- 0.06 ng/ml and 1.45 +/- 0.05 ng/ml for collagenase, 12 +/- 5 ng/ml and 22 +/- 9.5 ng/ml for TIMP-1, and 10.4 +/- 7.1 pr/ml and 14.11 +/- 6.1 pr/ml for TNF-alpha, respectively. Postoperative changes in the levels of collagenase (p=0.098) and TNF-alpha (p=0.069) were not significant, whereas increase in the TIMP-1 level was significant (p=0.026). Conclusion: This study showed elevated levels of TNF-alpha, collagenase, and TIMP-1 due to the presence of ruptured ACL. Our findings showed how these levels changed in the acute postoperative period.eninfo:eu-repo/semantics/openAccessAnterior cruciate ligament/injuriesKnee jointMatrix metalloproteinasesSynovial fluidTissue inhibitor of metalloproteinasesTumor necrosis factor-alphaSynovial-fluid cytokineMatrix metalloproteinasesTissue inhibitorsOsteoarthritisTendonKneeInjuryTunnelJointDogsOrthopedicsAdolescentAdultAnterior cruciate ligamentCollagenasesFemaleHumansMaleMatrix metalloproteinasesMonitoring, intraoperativePostoperative periodReconstructive surgical proceduresRupture, spontaneousSynovial fluidTissue inhibitor of metalloproteinase-1Tumor necrosis factor-alphaYoung adultArticle0002690831000052-s2.0-7764919500521421843319717938OrthopedicsTissue Inhibitor of Metalloproteinase-1; Gelatinase A; Matrix Metalloproteinase InhibitorsCollagenaseMatrix metalloproteinaseTissue inhibitor of metalloproteinase 1Tumor necrosis factor alphaAdolescentAdultAnterior cruciate ligamentArticleEnzymologyFemaleHumanInjuryMaleMetabolismMethodologyPatient monitoringPlastic surgeryPostoperative periodRuptureSynovial fluid