Akgün, EmelÇevik, ÖzgeBaykal, Ahmet TarıkTanaka, RyouCeron, Jose Joaquin2024-02-072024-02-072020-11-30Levent, P. vd. (2020). "Platelet proteome changes in dogs with congestive heart failure". BMC Veterinary Research, 16(1).https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02692-xhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708215/https://hdl.handle.net/11452/39584Background: Platelets play a central role in the development of cardiovascular diseases and changes in their proteins are involved in the pathophysiology of heart diseases in humans. There is lack of knowledge about the possible role of platelets in congestive heart failure (CHF) in dogs. Thus, this study aimed to investigate the changes in global platelet proteomes in dogs with CHF, to clarify the possible role of platelets in the physiopathology of this disease. Healthy-dogs (n = 10) and dogs with acute CHF due to myxomatous mitral valve disease (MMVD, n = 10) were used. Acute CHF was defined based on the clinical (increased respiratory rate or difficulty breathing) and radiographic findings of pulmonary edema. Dogs Blood samples were collected into tubes with acid-citrate-dextrose, and platelet-pellets were obtained by centrifuge and washing steps. Platelet-proteomes were identified using LC-MS based label-free differential proteome expression analysis method and matched according to protein database for Canis lupus familiaris. Results: Totally 104 different proteins were identified in the platelets of the dogs being 4 out of them were significantly up-regulated and 6 down-regulated in acute CHF dogs. Guanine-nucleotide-binding protein, apolipoproteins (A-II and C-III) and clusterin levels increased, but CXC-motif-chemokine-10, cytochrome-C-oxidase-subunit-2, cathepsin-D, serine/threonine-protein-phosphatase-PP1-gamma-catalytic-subunit, creatine-kinase-B-type and myotrophin levels decreased in acute CHF dogs. These proteins are associated with several molecular functions, biological processes, signaling systems and immune-inflammatory responses. Conclusion: This study describes by first time the changes in the protein composition in platelets of dogs with acute CHF due to MMVD. Our findings provide a resource for increase the knowledge about the proteome of canine platelets and their roles in CHF caused by MMVD and could be a tool for further investigations about the prevention and treatment of this disease.eninfo:eu-repo/semantics/openAccessPlatelet proteomicHeart failureMyxomatous mitral valve diseaseApolipoprotein C-IIICardiac-hypertrophyAngiotensin-IIA-IIActivationMyotrophinDiseaseBiomarkersPhosphataseMortalityVeterinary sciencesDogsAnimalsBlood plateletsDog diseasesDogsFemaleHeart failureHeart valve diseasesMaleProteomeArticle0005964998000032-s2.0-8509694056416133256720https://doi.org/10.1186/s12917-020-02692-xVeterinary sciencesPimobendan; Mitral Valve Disease; Cavalier King Charles Spaniel4 (2 hydroxyethyl) 1 piperazineethanesulfonic acidAcetonitrileAcid citrate dextroseActinApolipoprotein AApolipoprotein CCathepsin DCitrate trisodiumCitric acidClusterinCreatine kinaseCyclic ampDependent protein kinaseCyclooxygenase 1 inhibitorCyclooxygenase 2 inhibitorCytochrome BCytochrome C oxidaseDipeptidyl carboxypeptidase inhibitorDiuretic agentFibrinopeptide BFibronectinGamma interferon inducible protein 10Gelatinase BGlucoseGuanine nucleotide binding proteinHeat shock proteinInotropic agentMagnesium sulfateMetalloproteinase inhibitorPotassium chlorideProstaglandin e1ProteomeRecombinant somatomedin CSodium chlorideTroponin LProteomeAnimal experimentAnimal modelArticleAtrial fibrillationBreathing rateCell countingColor boppler echocardiographyCongestive heart failureControlled studyCoughingDogDown regulationDyspneaEchocardiographyElectrocardiographyExercise toleranceFemaleHeart atrium enlargementHeart ejection fractionHeart failureHeart left ventricle contractionHeart left ventricle enddiastolic volumeHeart rateHeart rhythmHeart valveHeart ventricle hypertrophyHeart ventricle septum ruptureHeart ventricle sizeLeft ventricular fractional shorteningLiquid chromatography-mass spectrometryLung congestionLung edemaLung infiltrateMaleMitral valve diseaseMitral valve regurgitationNonhumanPleura effusionProspective studyProtein analysisProtein expressionProtein interactionRespiratory distressReversed phase liquid chromatographyScoring systemSignal transductionSinus tachycardiaSystolic heart murmurThorax radiographyThrombocyteTime of flight mass spectrometryTransthoracic echocardiographyUpregulationVertebral heart scoreAnimalBloodDogDog diseaseHeart failureMetabolismPathophysiologyThrombocyteValvular heart diseaseVeterinary medicine1746-6148