Bharali, Dhruba J.Davis, Paul J.Mousa, Shaker A.2022-08-192022-08-192013-12Bharali, D. J. vd. (2013). "Tetraiodothyroacetic acid-conjugated PLGA nanoparticles: A nanomedicine approach to treat drug-resistant breast cancer". Nanomedicine, 8(12), 1943-1954.1743-58891748-6963https://doi.org/10.2217/nnm.12.200https://www.futuremedicine.com/doi/10.2217/nnm.12.200http://hdl.handle.net/11452/28277Aim: The aim was to evaluate tetraiodothyroacetic acid (tetrac), a thyroid hormone analog of l-thyroxin, conjugated to poly(lactic-co-glycolic acid) nanoparticles (T-PLGA-NPs) both in vitro and in vivo for the treatment of drug-resistant breast cancer. Materials & methods: The uptake of tetrac and T-PLGA-NPs in doxorubicin-resistant MCF7 (MCF7-Dx) cells was evaluated using confocal microscopy. Cell proliferation assays and a chick chorioallantoic membrane model of FGF2-induced angiogenesis were used to evaluate the anticancer effects of T-PLGA-NPs. In vivo efficacy was examined in a MCF7-Dx orthotopic tumor BALBc nude mouse model. Results: T-PLGA-NPs were restricted from entering into the cell nucleus, and T-PLGA-NPs inhibited angiogenesis by 100% compared with 60% by free tetrac. T-PLGA-NPs enhanced inhibition of tumor-cell proliferation at a low-dose equivalent of free tetrac. In vivo treatment with either tetrac or T-PLGA-NPs resulted in a three- to five-fold inhibition of tumor weight. Conclusion: T-PLGA-NPs have high potential as anticancer agents, with possible applications in the treatment of drug-resistant cancer. Original submitted 2 May 2012; Revised submitted 21 November 2012eninfo:eu-repo/semantics/openAccessBiotechnology & applied microbiologyScience & technology - other topicsAngiogenesisBreast cancerChick chorioallantoic membraneMCF7 breast cancer cellNanoparticleTetracThyroid hormoneCell-surface receptorThyroid-hormoneGrowth-factorBiodegradable nanoparticlesQuantum DotsIn-vitroDeliveryChitosanMicroparticlesCarcinomaAnimalsAntineoplastic agentsBreastBreast neoplasmsCell line, tumorCell proliferationDoxorubicinDrug resistance, neoplasmFemaleHumansLactic acidMiceMice, inbred BALB CMice, nudeNanoparticlesNeovascularization, pathologicPolyglycolic acidThyroxineArticle0003273796000082-s2.0-848874084741943195481223448245Biotechnology & applied microbiologyNanoscience & nanotechnologyIntegrin; Thyroid Hormones; Nano-Diamino-TetracFibroblast growth factor 2Integrin receptorNanoparticlePolyglactinTetraiodothyroacetic acidThyroid hormoneUnclassified drugAngiogenesisAnimal cellAnimal experimentAnimal modelArticleBreast cancerCancer cellCell nucleusCell proliferationCellular distributionChorioallantoisConfocal microscopyControlled studyFemaleMouseNanomedicineNonhumanPriority journalTumor cell