Maan, Narender SinghMaan, SushilaNomikou, KyriakiJohnson, Donna J.El-Harrak, MehdiMadani, HafsaYadin, Hagaiİnçoğlu, ŞerifeAllison, Andrew B.Stallknecht, David E.Batten, Carrie AnneAnthony, Simon JohnMertens, Peter Paul Clement2022-09-142022-09-142010-09-17Maan, N. S. vd. (2010). "RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the mediterranean region and North America". Plos One, 5(9), 1-13.1932-6203https://doi.org/10.1371/journal.pone.0012782https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0012782http://hdl.handle.net/11452/28721Epizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assays. EHDV outer-capsid protein VP2 (encoded by genomesegment 2 [Seg-2]) is highly variable and represents the primary target for neutralising antibodies generated by the mammalian host. Consequently VP2 is also the primary determinant of virus "serotype", as identified in virus neutralisation tests (VNT). Although previous reports have indicated eight to ten EHDV serotypes, recent serological comparisons and molecular analyses of Seg-2 indicate only seven EHDV "types". Oligonucleotide primers were developed targeting Seg-2, for use in conventional RT-PCR assays to detect and identify these seven types. These assays, which are more rapid and sensitive, still show complete agreement with VNT and were used to identify recent EHDV isolates from the Mediterranean region and North America.eninfo:eu-repo/semantics/openAccessWhite-tailed deerPolymerase chain-reactionBluetongue-virusCompetitive elisaIbaraki-virusSequence-analysisGenomeDifferentiationAntibodiesProteinsScience & technology - other topicsBosEpizootic hemorrhagic disease virusMammaliaMiridaeAnimalsCapsid proteinsCattleCattle diseaseCell lineCricetinaeHemorrhagic disease virus, epizooticMediterranean regionMolecular sequence dataNorth americaPhylogenyReoviridae infectionsReverse transcriptase polymerase chain reactionArticle0002819608000112-s2.0-7795856735311359Multidisciplinary sciencesSerotype; Epizootic Hemorrhagic Disease Virus; African Horse Sickness VirusNeutralizing antibodyOligonucleotideProtein VP2Capsid proteinArticleControlled studyMammalNonhumanNorth AmericaNucleotide sequenceOrbivirusProtein targetingReverse transcription polymerase chain reactionSeg 2 geneSerologySerotypeSouthern EuropeVirus geneVirus genomeVirus isolationVirus neutralizationVirus strainVirus typingAnimalsAnimal diseaseCattleCattle diseaseCell lineClassificationEvaluationGeneticsHamsterIsolation and purificationMethodologyMolecular geneticsPhylogenyReovirus infectionVirology