Eskiler, Gamze Güney2023-10-182023-10-182018-10Eryılmaz, I. E. vd. (2018). ''In vitro cytotoxic and antiproliferative effects of usnic acid on hormone-dependent breast and prostate cancer cells''. Journal of Biochemical and Molecular Toxicology, 32(10).1095-66701099-0461https://doi.org/10.1002/jbt.22208https://onlinelibrary.wiley.com/doi/10.1002/jbt.22208http://hdl.handle.net/11452/34431The aim of the current study was first to investigate cytotoxic activity of usnic acid (UA) on hormone-dependent breast and prostate cancer, and normal cells. Cells were treated with increasing concentrations (25 to 150 mu M) of UA for 48hours and cell viability, quantitative and morphological analysis of cell death, and cell cycle analysis were performed. UA was shown to have selective cytotoxicity on hormone-dependent cancer cells with the IC50 levels of 71.4 and 77.5 mu M for MCF7 and LNCaP cells, respectively. UA induced apoptotic cell death and G0/G1 cell cycle arrest without damaging normal cells. MCF7 cells were more sensitive to UA than LNCaP cells. Our results first revealed that UA is a promising candidate as an alternative agent for hormone-dependent breast and prostate cancers. However, molecular mechanism underlying the UA-mediated cell death in cancer cells should be investigated further.eninfo:eu-repo/semantics/closedAccessBiochemistry & molecular biologyToxicologyApoptosisBreast cancerHormone-dependent cancersProstate cancerUsnic acidAdjuvant tamoxifenL-methionineDna-damageTherapyReceptorsLncapMcf-7MechanismsApoptosisCompoundAntineoplastic agentsApoptosisBenzofuransBreast neoplasmsCell lineCell line, tumorCell proliferationFemaleG1 phaseHumansInhibitory concentration 50MaleNeoplasms, hormone dependentProstatic neoplasmsResting phase, cell cycleArticle0004475517000092-s2.0-85052431648321030101414Biochemistry & molecular biologyToxicologyUsnic Acid; Extract; Lichen (Disease)HormoneUsnic acidAntineoplastic agentBenzofuran derivativeUsnic acidAntiproliferative activityApoptosisArticleBreast cancerCancer cellCell cycle regulationCell proliferationCell viabilityControlled studyCytotoxicityHumanHuman cellIC50In vitro studyLNCaP cell lineMCF-7 cell linePercentage of cells in G0/G1 phaseProstate cancerBreast tumorCell cycle G0 phaseCell cycle G1 phaseCell lineDrug effectFemaleMaleNeoplasmPathologyProstate tumorTumor cell line