2022-10-272022-10-272015-11-15Üstüner, B. vd. (2016). "Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender". Animal Reproduction Science, 164, 97-104.0378-43201873-2232https://doi.org/10.1016/j.anireprosci.2015.11.017https://www.sciencedirect.com/science/article/pii/S0378432015300610http://hdl.handle.net/11452/29227The aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in egg yolk or lecithin extender containing no RTSP, 0.1%, 1% or 10% RTSP (v/v). Semen samples were assessed for sperm motility, plasma membrane integrity [hypoosmotic swelling test (HOST) and Hoechst 33258] and defective acrosomes [FITC-conjugated Pisum sativum agglutinin (PSA-FITC)] at the following five time points: after dilution with extender A; after equilibration; and post-thaw at 0 h, 3 h and 5 h. Malondialdehyde (MDA) was examined only after thawing. Freezing and thawing procedures (dilution, equilibration and post-thaw incubation at Oh, 3 h and 5 h) negatively affected the motility (P<0.001) and acrosome integrity (P<0.001). Additionally, freezing and thawing negatively affected the plasma membrane integrity, as determined by the HOST and Hoechst 33258 (P<0.001). The extender group affected the motility (P<0.001) and the HOST results (P<0.001). Levels of MDA in the egg yolk extender with 1% RTSP group were significantly lower than in the lecithin control group (P<0.05). In conclusion, the egg yolk extender groups that were supplemented with 10% and 1% RTSP provided greater cryoprotective effects for semen survivability during 5 h incubation than the other extender groups.eninfo:eu-repo/semantics/closedAccessAgricultureReproductive biologyVeterinary sciencesRam semenCryopreservationRainbow troutSeminal plasmaHypoosmotic swelling testIn-vitroCold-shockSpermSpermatozoaParametersFertilityCapacitationUnfrozenGlycerolAcrosomeAnimalsCell membraneCryopreservationCryoprotective agentsEgg yolkMaleMalondialdehydeOncorhynchus mykissPlant lectinsSemenSemen preservationSheepSoybean proteinsArticle0003695629000132-s2.0-849550761049710416426685096Agriculture, dairy & animal scienceReproductive biologyVeterinary sciencesExtender; Semen; Semen ExtendersCryoprotective agentMalonaldehydePlant lectinSoybean lectinSoybean proteinAcrosomeAnimalCell membraneCryopreservationEgg yolkMaleOncorhynchus mykissPhysiologySheepSpermSperm preservationVeterinary