Dimas, KonstantinosSakellaridis, Nikos2021-11-162021-11-162011-10Ulukaya, E. vd. (2011). " Cell death-inducing effect of novel palladium(II) and platinum(II) complexes on non-small cell lung cancer cells in vitro ". Journal of Cancer Research and Clinical Oncology, 137(10),1425-1434.0171-5216https://doi.org/10.1007/s00432-011-1021-1https://link.springer.com/article/10.1007/s00432-011-1021-1http://hdl.handle.net/11452/22667Purpose Treatment for lung cancer is still far from satisfying rates. Therefore, there is a need for novel anticancer agents. For this purpose, novel platinum and palladium complexes {[Pd(sac)(terpy)](sac).4H(2)O (Complex 1), [Pt(sac)(terpy)] (sac).5H(2)O (Complex 2), [PdCl(terpy)](sac).2H(2)O (Complex 3), [PtCl(terpy)](sac).2H(2)O (Complex 4)} have been tested against three different non-small cell lung cancer cell lines (A549, H1299, PC-3). Methods Growth-inhibiting effcts have been tested by the MTT and ATP viability assays. Apoptosis has been detected by the caspase-cleaved cytokeratin 18 (M30-antigen) assay. Necrosis has been detected by staining the cells with fluorescent dyes. Mitotic index has been calculated by counting the mitotic figures after staining with hematoxylin. Results The complex 3 exhibited significant anti-growth effects, and its anti-growth effect was more powerful than that of cisplatin that is a standard chemotherapeutic agent for this type of cancer. The complexes did not induce apoptosis, while necrosis clearly took place. Conclusions Novel Pd(II) complex ([PdCl(terpy)] (sac).2H(2)O) seems to represent a potentially active drug against non-small cell lung cancer cell lines, and further studies in vivo are warranted.eninfo:eu-repo/semantics/closedAccessOncologyLung cancer treatmentPalladium(II) and platinum(II) complexesNecrosisCytotoxic activityCrystal-structureAntitumor agentsCisplatinPd(II)DnaApoptosisPt(II)AssayHepatotoxicityAdenosine triphosphateApoptosisCarcinoma, non-small-cell lungCell line, tumorCisplatinHumansLung neoplasmsMitosisNecrosisOrganometallic compoundsOrganoplatinum compoundsPalladiumArticle0002959888000032-s2.0-80054759797142514341371021809029OncologyBenzisothiazole; Palladium; 2-Phenylpyridine3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromideAdenosine triphosphateCaspaseCisplatinCytokeratin 18Fluorescent dyeHematoxylinPalladium complexPlatinum complexAntineoplastic activityAnimal cellApoptosisArticleCancer cell cultureCancer inhibitionCell deathCell viabilityConcentration responseControlled studyDrug effectDrug potencyDrug structureEnzyme modificationHumanHuman cellIC 50In vitro studyLung non small cell cancerMitosis indexNonhumanPriority journal