İnal, Esra ErkolGörükmez, OrhanEroğlu, SelmaSolak, Özlem2023-05-162023-05-162015-06-27İnal, E. E. vd. (2016). "Association of GSTM1, GSTT1, GSTP1-ILE105VAL and ACE I/D polymorphisms with ankylosing spondylitis". Rheumatology International, 36(1), 17-23.0172-81721437-160Xhttps://doi.org/10.1007/s00296-015-3317-yhttps://link.springer.com/article/10.1007/s00296-015-3317-yhttp://hdl.handle.net/11452/32676Ankylosing spondylitis (AS) is a chronic inflammatory disease of unknown origin. The aim of this study is to clarify the relationships between susceptibility and severity of AS and GST-mu1 (GSTM1), GST-theta1 (GSTT1), GST-pi1 (GSTP1)-Ile105Val and angiotensin-converting enzyme (ACE) I/D polymorphisms in AS patients. One hundred thirty-eight AS patients and seventy-one healthy controls were enrolled in this study. Erythrocyte sedimentation rate and C-reactive protein (CRP) levels of the AS patients were recorded. The scores of the numeric rating scale (NRS) pain, the Bath Ankylosing Spondylitis Activity Index, the Bath Ankylosing Spondylitis Metrology Index and the Bath Ankylosing Spondylitis Functional Index were calculated. The genotypes distributions and allele frequencies of GSTM1, GSTT1, GSTP1-Ile105Val and ACE I/D polymorphisms were compared between patients and healthy controls. The Multiplex polymerase chain reaction (PCR) and the PCR-restriction fragment length polymorphism methods were used to detect the polymorphisms of ACE I/D, the GSTT1 and GSTM1 genes and the GSTP1-Ile105Val polymorphism, respectively. There were significantly higher levels of the GSTT1 null and the ACE II genotypes in AS patients compared to those in healthy controls (p = 0.002 and 0.005, respectively). We found significantly higher levels of CRP and the NRS pain scores in the patients with ACE ID or DD genotypes compared to those in the patients with ACE II genotypes (p = 0.005 and 0.035, respectively). The present results showed that genes involved in protection from oxidative stress and ACE gene may influence disease development and course in AS.eninfo:eu-repo/semantics/closedAccessRheumatologyGSTM1GSTT1GSTP1-Ile105ValACE I/DDisease activityDisease severityConverting enzyme geneInsertion-deletion polymorphismRheumatoid-arthritisSusceptibilityPrevalenceM1T1P1BathAdultAllelesC-reactive proteinCase-control studiesFemaleGene frequencyGenetic association studiesGenetic predisposition to diseaseGenotypeGlutathione S-transferase piGlutathione transferaseHumansMaleMiddle agedOxidative stressPeptidyl-dipeptidase APolymorphism, single nucleotideSpondylitis, ankylosingArticle0003678085000032-s2.0-84953836295172336126186891RheumatologyAnterior Cruciate Ligament; Gene; TendinitisC reactive proteinIsoleucineValineDipeptidyl carboxypeptidaseGlutathione S-transferase M1Glutathione S-transferase T1Glutathione transferaseGlutathione transferase P1GSTP1 protein, humanACE geneAdultAnkylosing spondylitisArticleBath ankylosing spondylitis activity indexBath ankylosing spondylitis functional indexBath ankylosing spondylitis metrology indexControlled studyDisease courseDisease severityErythrocyte sedimentation rateFemaleGeneGene frequencyGenetic associationGenetic polymorphismGenetic susceptibilityGenotypeGSTM1 geneGSTP1 geneGSTT1 geneHumanMajor clinical studyMaleMultiplex polymerase chain reactionOxidative stressPriority journalRating scaleRestriction fragment length polymorphismTurk (people)AlleleAnkylosing spondylitisBloodCase control studyGenetic association studyGenetic predispositionGeneticsMetabolismMiddle agedSingle nucleotide polymorphism