Mousa, Shaker A.Bharali, Dhruba J.Meng, RanTang, Heng-YuanLin, Hung-YunDavis, Faith B.Davis, Paul J.2021-11-302021-11-302012-04Mousa, S. A. vd. (2012). "Tetraiodothyroacetic acid and its nanoformulation inhibit thyroid hormone stimulation of non-small cell lung cancer cells in vitro and its growth in xenografts". Lung Cancer, 76(1), 39-45.0169-50021872-8332https://doi.org/10.1016/j.lungcan.2011.10.003https://pubmed.ncbi.nlm.nih.gov/22024450/https://www.sciencedirect.com/science/article/pii/S0169500211005162http://hdl.handle.net/11452/22888Thyroid hormone stimulates cell proliferation of several types of cancers and stimulates cancer-relevant angiogenesis. In the present study, we investigated the proliferative effect of thyroid hormone and the anti-proliferative and anti-angiogenic action of its nano-derivative, tetrac-NP, on human non-small cell lung cancer (NSCLC) H1299 cells in vitro and in xenografts. The anti-proliferative activity of unmodified tetrac and tetrac-NP against human H1299 cells was determined in three models: (a) cultured H1299 cells in vitro, (b) tumor cell implants in the fertilized chick chorioallantoic membrane (CAM) system and (c) xenografts in the nude mouse. An integrin alpha v beta 3 antibody inhibited thyroid hormone-induced cell proliferation in vitro, as did unmodified tetrac and tetrac-NP. Pharmacologic inhibition of the mitogen-activated protein kinase pathway also blocked NSCLC cell proliferation in response to thyroid hormone. Tetrac and tetrac-NP arrested tumor growth and tumor-related angiogenesis in H1299 cells grown in the CAM model and both agents prevented chick embryo mortality. Xenografts of H1299 cells were established in nude mice (n = 8, treatment and control groups) and when tumor volumes reached 250-300 mm(3), tetrac (1 mg/kg) or tetrac-NP (1 mg tetrac as the nanoparticle/kg) were administered intraperitoneally every 2 days. Tetrac and tetrac-NP significantly suppressed tumor growth and angiogenesis. Thus, both tetrac and tetrac-NP effectively arrest human NSCLC tumor cell proliferation in vitro and in the CAM assay and in murine xenograft models.eninfo:eu-repo/semantics/closedAccessOncologyRespiratory systemNon-small cell lung cancerThyroid hormoneTetracTetrac nanoparticlesAngiogenesisCell surface integrin alpha v beta 3 receptorCancerActivated protein-kinaseSurface receptorL-thyroxineIntegrinHypothyroidismCarcinomaGlioma3,5,3'-triiodo-l-thyronineProliferationAnimalsCarcinoma, non-small-cell lungCell line, tumorCell proliferationChick embryoChorioallantoic membraneFemaleHumansInjections, intraperitonealIntegrin alpha v beta 3Lung neoplasmsMiceMice, nudeNanoparticlesThyroid hormonesThyroxineXenograft model antitumor assaysArticle0003025846000052-s2.0-84858003654394576122024450OncologyRespiratory systemTetraiodothyroacetic Acid; Thyroid Hormones; Nano-Diamino-TetracLiothyronineMitogen activated protein kinaseNanoparticleTetraiodothyroacetic acidTetraiodothyroacetic acid nanoparticleThyromimetic agentThyroxineUnclassified drugVitronectin receptorAnimal cellAnimal experimentAnimal modelAntiangiogenic activityAntiproliferative activityArticleCancer cellCancer growthCancer inhibitionCancer sizeCell proliferationChickChorioallantoisControlled studyEnzyme inhibitionFemaleHormonal regulationHumanHuman cellIn vitro studyLung non small cell cancerMouseNonhumanPharmacological blockingPriority journalTreatment responseTumor xenograft