Zorluoǧlu, Abdullah2023-05-172023-05-172013-12Ak, S. vd. (2013). “Microsatellite instability status affects gene expression profiles in early onset colorectal cancer patients”. Journal of Surgical Research, 185(2), 626-637.0022-48041095-8673https://doi.org/10.1016/j.jss.2013.07.014http://hdl.handle.net/11452/32702Background: The association between microsatellite instability (MSI) status and gene expression profiles in the early onset sporadic colorectal cancer (CRC) has not been clearly established. The aim of this study was to identify the altered gene expression patterns depending on the MSI status of early onset CRC and determine specific biomarkers that could provide novel therapeutic molecular targets in the Turkish population. Materials and methods: MSI markers (BAT25, BAT26, D2S123, D5S346, and D17S250) were investigated in tumors from 36 early onset sporadic CRC patients in whom gene expression profiles were analyzed previously. The relationship between the gene expression profiles depending on MSI status was evaluated. Results: A total of 15 tumors (16.66%) were identified as having MSI and 21 tumors (58.33%) were identified as having microsatellite stability (MSS). CK20 and MAP3K8 upregulation, observed in MSS tumors, was significantly associated with lymph node metastasis, recurrence, and/or distant metastasis and a short median survival (P < 0.05). REG1A upregulation is also correlated with recurrence and/or distant metastasis and a short median survival in patients with MSI tumors (P < 0.05). Conclusions: High expression levels of CK20 and MAP3K8 in MSS tumors and REG1A in MSI tumors correlated with a poor prognosis in CRC patients. Further studies and validations are required; these genes may provide novel therapeutic molecular targets for the development of anticancer drugs related to MSI status for early onset CRC treatment.eninfo:eu-repo/semantics/closedAccessSurgeryColorectal cancerMSIMSSCK20MAP3K8REG1AEarly onsetMessenger-rna expressionReg-ivcytokeratin-20CarcinomasOverexpressionIdentificationChemotherapyAlphaDnaDifferentiationColorectal cancerEarly onsetAdolescentAdultAge of onsetColorectal neoplasmsDNA methylationFemaleGene expression regulation, neoplasticGenetic markersGenetic testingHumansKeratin-20LithostathineLymphatic metastasisMaleMAP kinase kinase kinasesMicrosatellite instabilityMiddle agedPrognosisProto-oncogene proteinsTranscriptomeYoung adultArticle0003269385000472-s2.0-84891668986626637185223992855SurgeryInferior Mesenteric Artery; Complete; Lymph Node DissectionBiological markerCytokeratin 20LithostathineMAP3K8 protein, humanMitogen activated protein kinase kinase kinaseOncoproteinREG1A protein, humanTranscriptomeGenetic markerAdultArticleBAT25 geneBAT26 geneCancer patientCancer prognosisCancer recurrenceCarcinogenesisCK20 geneClinical articleColorectal cancerColorectal carcinogenesisControlled studyD17S250 geneD2S123 geneD5S346 geneDistant metastasisFemaleFollow upGeneGene expression profilingHeredityHumanHuman tissueLymph node metastasisMaleMAP3K8 geneMicrosatellite instabilityMicrosatellite markerMicrosatellite stabilityMiddle agedPopulation genetic parametersPriority journalREG1A geneSurvivalTurkey (republic)UpregulationYoung adultAdolescentCK20Colorectal cancerColorectal tumorDNA methylationEarly onsetGene expression regulationGenetic markerGenetic screeningGeneticsLymph node metastasisMAP3K8MetastasisMortalityMSIMSSOnset agePrognosisREG1AColorectal tumorSecondary