Özel, Mustafa ZaferFırat, Mehmet2024-10-222024-10-222023-01-011516-8913https://doi.org/10.1590/1678-4324-2023220358https://hdl.handle.net/11452/46811Breast cancer is the leading cause of cancer-related deaths in women throughout the world. Research on natural anti-cancer products from plants has gained traction. Salvia L. species and their derivatives are rare in Turkey and have suggested for their potential anti-cancer effects. The aim of this study is to assess the potential cytotoxic/apoptotic activities of methanol extract of Salvia candidissima Vahl. subsp. candidissima (SCE) on MCF-7 and MDA-MB-231 breast cancer cells. A GCxGC-TOF/MS system and a dual stage commercial thermal desorption injector were used to determine the chemical components of SCE. MTT and ATP viability tests were used to investigate the anti-growth activity. The apoptosis-inducing effect was assessed using a fluorescence staining method. Caspase-cleaved keratin 18 (ccK18, M30-antigen) levels measured by M30-CytoDeath ELISA Kit. The results showed that SCE suppressed the survival of the MCF-7 and MDA-MB-231 breast cancer cells in a dose-dependent manner, based on the findings of both MTT and ATP cell viability tests and pyknotic cell nuclei were observed via fluorescent staining in both cell lines after 48 h of treatment. The treatment group had greater levels of caspase-cleaved keratin 18 in the MCF-7 cells than the untreated group. These results showed that SCE triggers apoptosis, causes cell death in MCF-7 and MDA-MB-231 cell lines. SCE may become promising therapeutic strategy in the treatment of breast cancer with further in vitro and in vivo studies.eninfo:eu-repo/semantics/closedAccessBeta-caryophylleneEssential oilChemical-compositionGrowth-inhibitionMyrica-rubraApoptosisRootsAntioxidantInductionSclareolSalvia candidissima vahlSubspCandidissimaBreast cancerMcf-7Mda-mb-231CytotoxicityScience & technologyLife sciences & biomedicine - other topicsBiologyArticle0010196979000016610.1590/1678-4324-2023220358