Bilir, Ayhan2023-10-132023-10-132015-03Tezcan, G. vd. (2015). "Ficus carica latex prevents invasion through induction of let-7d expression in GBM cell lines". Cellular and Molecular Neurobiology, 35(2), 175-187.0272-4340https://doi.org/10.1007/s10571-014-0109-yhttps://link.springer.com/article/10.1007/s10571-014-0109-yhttp://hdl.handle.net/11452/34334Glioblastoma multiforme (GBM) is one of the deadliest human malignancies. A cure for GBM remains elusive, and the overall survival time is less than 1 year. Thus, the development of more efficient therapeutic approaches for the treatment of these patients is required. Induction of tumor cell death by certain phytochemicals derived from medicinal herbs and dietary plants has become a new frontier for cancer therapy research. Although the cancer suppressive effect of Ficus carica (fig) latex (FCL) has been determined in a few cancer types, the effect of this latex on GBM tumors has not been investigated. Therefore, in the current study, the anti-proliferative activity of FCL and the effect of the FCL-temozolomide (TMZ) combination were tested in the T98G, U-138 MG, and U-87 MG GBM cell lines using the WST-1 assay. The mechanism of cell death was analyzed using Annexin-V/FITC and TUNEL assays, and the effect of FCL on invasion was tested using the chick chorioallantoic membrane assay. To determine the effect of FCL on GBM progression, the expression levels of 40 GBM associated miRNAs were analyzed in T98G cells using RT-qPCR. According to the obtained data, FCL causes cell death in GBM cells with different responses to TMZ, and this effect is synergistically increased in combination with TMZ. In addition, the current study is the first to demonstrate the effect of FCL on modulation of let-7d expression, which may be an important underlying mechanism of the anti-invasive effect of this extract.eninfo:eu-repo/semantics/closedAccessCell biologyNeurosciences & neurologyFicus carica latexGlioblastomaTemozolomideMicroRNAInvasionGene-expressionProtocatechuic acidFig treeTGF-betaCancerGrowthStat3MetastasisIdentificationProliferationFicus caricaAntineoplastic agentsApoptosisBrain neoplasmsCell line, tumorCell proliferationCell survivalChemical fractionationDacarbazineFicusGene expression regulation, neoplasticGlioblastomaHumansIn situ nick-end labelingLatexMicroRNAsModels, biologicalNeoplasm invasivenessNeovascularization, physiologicPhytotherapyPlant extractsArticle0003502368000042-s2.0-8492353229217518735225212824Cell biologyNeurosciences & neurologyExtract; Moraceae; Cyanidin 3-rutinosideAntineoplastic agentCaffeic acidFerulic acidFicus carica latexLatexMicroRNAMicroRNA let 7dPara coumaric acidProtocatechuic acidQuercetinTemozolomideUnclassified drugVasculotropinAntineoplastic agentDacarbazineLatexMirnlet7 microRNA, humanPlant extractTemozolomideAdultAntineoplastic activityAntiproliferative activityApoptosisArticleCell viabilityChorioallantoisControlled studyCytotoxicityDrug mechanismDrug potentiationFemaleFigFractionationGene expressionGlioblastoma cell lineHigh performance liquid chromatographyHumanHuman cellMaleNick end labelingNonhumanNormal humanPriority journalAnalogs and derivativesAngiogenesisBiological modelBrain tumorCell proliferationCell survivalChemistryDrug effectsFicusGene expression regulationGeneticsGlioblastomaMetabolismPathologyPhytotherapyTumor cell lineTumor invasionTUNEL assay