Saraç-Pektaş, Fatma2024-09-112024-09-112023-12-012667-646Xhttps://doi.org/10.36519/idcm.2023.291https://hdl.handle.net/11452/44599The diagnosis of Lyme disease is becoming more common in Turkey. Nonetheless, some physicians are not aware of the diagnostic principles that should be followed when faced with a suspected patient and could use tests that are not recommended, such as darkfield microscopy. Dark field microscopy is a diagnostic technique to visualize the spirochetes that cause Lyme disease; however, it is not recommended for the diagnosis of Lyme disease. One of the main limitations of dark field microscopy is its low sensitivity. Another limitation is its high false -positivity rate, as other microorganisms and cellular debris can be mistaken for spirochetes, leading to a misdiagnosis that may result in unnecessary treatment. Therefore, this study aimed to review the literature on the role of dark field microscopy as a diagnostic method for Lyme disease and inform physicians about recommended approaches in line with the recommendations of national or international guidelines. An electronic search of Pubmed, Scopus, and Web of Science was performed using the following medical subject headings (MeSH) search terms: Lyme borreliosis, Lyme disease, Borrelia burgdorfen, diagnosis, and microscopy. With this narrative review, we aimed to inform physicians better and improve patient care for patients with suspected Lyme disease.eninfo:eu-repo/semantics/openAccessBorrelia-burgdorferiUnited-statesBabesiaLyme diseaseLyme borreliosisDark field microscopyDiagnosisScience & technologyLife sciences & biomedicineMicrobiologyInfectious diseasesReview0011578848000082812865410.36519/idcm.2023.291