İnci, DuyguAydın, RahmiyeZorlu, Yunus2024-11-122024-11-122021-02-050022-2860https://doi.org/10.1016/j.molstruc.2020.129099https://www.sciencedirect.com/science/article/pii/S0022286020314216https://hdl.handle.net/11452/47741Two water soluble copper(II) mono-anionic glutamatecomplexes-[Cu(phen)(glu)(H2O)]NO3<bold>H</bold>2O (1) and [Cu(py-phen)(glu)(H2O)]NO3<bold>H</bold>2O (2) (phen: 1,10-phenanthroline, py-phen: pyrazino[2,3-f][1,10]phenanthroline, glu: glutamic acid), have been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complex with biomacromolecules {calf thymus DNA (CT-DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy methods. The experimental outcomes displays that the complexes 1 and 2 bind to CT-DNA by means of a moderate intercalation mode. Furthermore, the fluorescence quenching mechanism between BSA and the complexes 1 and 2 is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (AG, AH, AS) of BSA + the complexes 1 and 2 systems were determined at different temperatures. The binding distance between BSA and the complexes 1 and 2 was calculated according to Forster non-radiation energy transfer theory (FRET). The effect of the complexes 1 and 2 on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Radical scavenging activity of the complexes 1 and 2 was determined in terms of IC50, using the DPPH and H2O2 method. In biomacromolecules interactions and radical activity studies, the complexes 1 and 2 were found to give good results. (C) 2020 Elsevier B.V. All rights reserved.eninfo:eu-repo/semantics/closedAccessSerum-albuminCopper(ii)Glutamic acid1,10-phenanthroline derivativesBiomacromolecular interactionsRadical scavenging activityScience & technologyPhysical sciencesChemistry, physicalChemistryArticle000595273300012122510.1016/j.molstruc.2020.1290991872-8014