Watkins, Carol J.Beek, Eline M.van derWurtman, Richard J.2021-07-052021-07-052005-10-05Cansev, M. vd. (2005). "Oral uridine-5 '-monophosphate (UMP) increases brain CDP-choline levels in gerbils". Brain Research, 1058(1-2), 101-108.0006-8993https://doi.org/10.1016/j.brainres.2005.07.054https://www.sciencedirect.com/science/article/pii/S0006899305011224http://hdl.handle.net/11452/21075We examined the biochemical pathways whereby oral uridine-5'-monophosphate (UMP) increases membrane phosphatide synthesis in brains of gerbils. We previously showed that supplementing PC12 cells with uridine caused concentration-related increases in CDP-choline levels, and that this effect was mediated by elevations in intracellular uridine triphosphate (UTP) and cytidine triphosphate (CTP). In the present study, adult gerbils received UMP (1 mmol/kg), a constituent of human breast milk and infant formulas, by gavage, and plasma samples and brains were collected for assay between 5 min and 8 It thereafter. Thirty minutes after gavage, plasma uridine levels were increased from 6.6 +/- 0.58 to 32.7 +/- 1.85 pM (P < 0.001), and brain uridine from 22.6 +/- 2.9 to 89.1 +/- 8.82 pmol/mg tissue (P < 0.001). UMP also significantly increased plasma and brain cytidine levels; however, both basally and following UMP, these levels were much lower than those of uridine. Brain UTP, CTP, and CDP-choline were all elevated 15 min after UMP (from 254 +/- 31.9 to 417 +/- 50.2, [ P < 0.05]; 56.8 +/- 1.8 to 71.7 +/- 1.8, [P < 0.001]; and 11.3 +/- 0.5 to 16.4 1, [P < 0.001] pmol/rng tissue, respectively), returning to basal levels after 20 and 30 min. The smallest UMP dose that significantly increased brain CDP-choline was 0.05 inmol/kg. These results show that oral UMP, a uridine source, enhances the synthesis of CDP-choline, the immediate precursor of PC, in gerbil brain. (c) 2005 Elsevier B.V All rights reserved.eninfo:eu-repo/semantics/closedAccessUMPUridineCytidineCDP-cholineCTPPCTransport-systemsBiosynthesisNeurite outgrowthChoroid-plexusUridine levelsNucleosideCytidinePlasmaPurineMiceNeurosciences & neurologyArticle0002328687000112-s2.0-2624446615210110810581-216126180Neurosciences