Zolini, A. M.Ortiz, W. G.Estrada-Cortes, E.Ortega, M. S.Sosa, F.Hansen, P. J.Giordano, Julio O.2024-05-272024-05-272019-010022-03021525-3198https://www.sciencedirect.com/science/article/pii/S0022030218310695https://hdl.handle.net/11452/41530Fertility-promoting effects of treatment of lactating dairy cattle with human chorionic gonadotropin (hCG) after artificial insemination (AI) have been variable. Here, we tested whether fertility response to hCG in lactating Holstein cows interacts with genotype and parity. Primiparous (n = 538) and multiparous (n = 613) cows were treated with hCG (3,300 IU) or vehicle 5 d after AI. Pregnancy was diagnosed on d 32 and 60 after AI. A subset of cows (n = 593-701) was genotyped for 4 single nucleotide polymorphisms (SNP) previously associated with fertility. Treatment with hCG increased progesterone concentration on d 12 after AI regardless of genotype or parity. Pregnancy per AI was improved by hCG in primiparous cows but riot in multiparous cows. Moreover, hCG treatment interacted with a SNP in coenzyme Q9 (COQ9) to affect fertility. Fertility of cows treated with vehicle was greatest for the AA allele, whereas fertility was lowest for the same genotype among cows treated with hCG. Pregnancy per AI was also affected by genotype for heat shock protein AI-like (HSPA1L) and progesterone receptor (PGR), but no interactions were observed with treatment. Genotype for a SNP in prostate androgen-regulated mucin-like protein 1 (PARM1) was not associated with fertility. Overall, results show that variation in response to hCG treatment on fertility depends on parity and interacts with a SNP in COQ9.eninfo:eu-repo/semantics/closedAccessHuman chorionic gonadotropinGenotypeParitySnpFertilitySingle nucleotide polymorphismsGenetic-variationProgesterone supplementationReproductive-performanceCyp2d6 polymorphismsCorpus-luteumCorpus-luteumAssociationTraitsEmbryoAnimalsCattleChorionic GonadotropinFemaleFertilityGenotypeHumansInsemination, ArtificialLactationParityPregnancyProgesteroneReceptors, ProgesteroneArticle0004541314000782-s2.0-85056636160846856102130447974https://doi.org/10.3168/jds.2018-15358Agriculture, dairy & animal scienceFood science & technologyArtificial Insemination; Progesterone; EstrusChorionic gonadotropinProgesteroneProgesterone receptorProgesterone receptor BAnimalArtificial inseminationBloodBovineDrug effectFemaleFertilityGeneticsGenotypeHumanLactationMetabolismParityPhysiologyPregnancyVeterinary medicine