2024-01-242024-01-242020-03-23Güvenç, G. B. vd. (2020). "Intracerebroventricularly injected nesfatin-1 activates central cyclooxygenase and lipoxygenase pathways". Autonomic Neuroscience-Basic and Clinical, 226.1566-0702https://www.sciencedirect.com/science/article/pii/S1566070220300199https://hdl.handle.net/11452/39300Nesfatin-1 is a multifunctional neuropeptide having crucial autonomic roles. It is well known that nesfatin-1 collaborates with other central neuromodulatory systems, such as central corticotropin-releasing hormone, melanocortin, oxytocin, and cholinergic systems to show its autonomic effects. Central arachidonic acid cascade plays an important role to provide the homeostasis by exhibiting similar autonomic effects to nesfatin-1. Based on these similarities, the current study was designed to show the effects of intracerebroventricularly (ICV) injected nesfatin-1 on the hypothalamic arachidonic acid (AA) cascade.Immunochemistry and western blot approaches demonstrated that ICV administration of nesfatin-1 provokes an increase in the hypothalamic cyclooxygenase (COX) -1, -2 and lipoxygenase (LOX) protein expression. Moreover, the microdialysis study demonstrated that centrally injected nesfatin-1 increased the posterior hypothalamic extracellular AA products.In conclusion, these findings report that while nesfatin-1 is generating its autonomic effects, it also might be using central prostaglandins and leukotrienes by activating central COX and LOX pathways.eninfo:eu-repo/semantics/closedAccessNesfatin-1CyclooxygenaseLipoxygenaseHypothalamusIntracerebroventricularCritical hemorrhagic hypotensionPhispholipase a(2)ActivatorEndogenus central histamineBrain thromboxane a(2)Arachidonic-acidNitric-oxideFood-intakeInvolvementSystemRatsNeurosciences & neurologyAnimalsArachidonic acidHypothalamusInjections, intraventricularLipoxygenasesMaleMicrodialysisNucleobindinsProstaglandin-endoperoxide synthasesProstaglandinsRatsRats, sprague-dawleySignal transductionArticle0005354712000052-s2.0-8508344199922632334147https://doi.org/10.1016/j.autneu.2020.102670NeurosciencesNucleobindin; Pyroglutamyl-Histidyl-Glycine; DNA-Binding ProteinsArachidonic acidCyclooxygenase 1Cyclooxygenase 2LipoxygenaseNesfatin 1NeuropeptideProstaglandin synthaseUnclassified drugArachidonic acidLipoxygenaseNucb2 protein, ratProstaglandinProstaglandin synthaseAnimal experimentAnimal tissueArticleAutonomic nervous systemControlled studyHypothalamusImmunochemistryMaleMicrodialysisNonhumanPlayPriority journalProtein expressionRatSignal transductionWestern blottingAdministration and dosageAnimalDrug effectIntracerebroventricular drug administrationMetabolismPharmacologySignal transductionSprague dawley rat1872-7484