Franco-Martínez, LorenaTvarijonaviciute, AstaHorvatić, AnitaGuillemin, NicolasCerón, José JoaquínEscribano, DamiánEckersall, DavidLamy, ElsaMartínez-Subiela, SilviaMrljak, Vladimir2022-12-282022-12-282018-09-18Franco-Martínez, L. vd. (2018). ''Changes in salivary analytes in canine parvovirus: A high-resolution quantitative proteomic study''. Comparative Immunology, Microbiology and Infectious Diseases, 60, 1-10.0147-95711878-1667https://doi.org/10.1016/j.cimid.2018.09.011https://www.sciencedirect.com/science/article/pii/S0147957118300742http://hdl.handle.net/11452/30129The present study evaluated the changes in salivary proteome in parvoviral enteritis (PVE) in dogs through a high-throughput quantitative proteomic analysis. Saliva samples from healthy dogs and dogs with severe parvovirosis that survived or perished due to the disease were analysed and compared by Tandem Mass Tags (TMT) analysis. Proteomic analysis quantified 1516 peptides, and 287 (corresponding to 190 proteins) showed significantly different abundances between studied groups. Ten proteins were observed to change significantly between dogs that survived or perished due to PVE. Bioinformatics' analysis revealed that saliva reflects the involvement of different pathways in PVE such as catalytic activity and binding, and indicates antimicrobial humoral response as a pathway with a major role in the development of the disease. These results indicate that saliva proteins reflect physiopathological changes that occur in PVE and could be a potential source of biomarkers for this disease.eninfo:eu-repo/semantics/closedAccessImmunologyMicrobiologyVeterinary sciencesBioinformaticsBiomarkerCanine parvovirosisDogLabel-based proteomicsSalivaHistidine-rich glycoproteinAcute-phase proteinsDogsBiomarkersInfectionIdentificationDiagnosisEnteritisLipocalinSerumAnimalsBiomarkersComputational biologyDog diseasesDogsEnteritisFemaleMaleParvoviridae infectionsParvovirus, canineProteomicsSalivaSalivary proteins and peptidesArticle0004490718000012-s2.0-850543384851106030396423ImmunologyMicrobiologyVeterinary sciencesCanine Parvovirus; Feline Panleukopenia Virus; DogAlpha actinin 1AmikacinAmpicillinApolipoprotein A1Biological markerBPI fold containing family B member 2 proteinCathelicidin antimicrobial peptideCD177 antigenClusterinCystatin MEF hand domain containing protein D2EzrinGelatinase BGlutathione derivativeHemoglobin beta chainImmunoglobulin heavy chainLactoperoxidaseLactoylglutathione lyaseLeukocyte elastaseLipocalin 9MetronidazolePlastin 2ProteomeRetinol binding protein 4Rho GDP dissociation inhibitor beta proteinSaliva proteinTrefoil factor 1Ubiquitin CUnclassified drugUnindexed drugVitamin D binding proteinBiological markerSaliva proteinArticleBioinformaticsCanine parvovirusCatalysisControlled studyDisease severityDogDown regulationFemaleHigh resolution quantitative proteomic analysisHigh throughput screeningHumoral immunityMaleNonhumanParvovirosisParvovirus infectionPathophysiologyProtein analysisProtein bindingProtein expressionProtein functionProteomicsQuantitative analysisSalivaSaliva analysisSurvivalTandem mass spectrometryTandem Mass Tags based proteomic analysisUpregulationAnimalBiologyCanine parvovirusChemistryDog diseaseEnteritisImmunologyIsolation and purificationMortalityParvovirus infectionProteomicsSalivaVeterinary medicineVirology