2023-09-192023-09-192017Özarda, Y. vd. (2017). ''A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood''. Biochemia Medica, 27(2), 350-377.1330-09621846-7482https://doi.org/10.11613/BM.2017.038https://www.biochemia-medica.com/en/journal/27/2/10.11613/BM.2017.038http://hdl.handle.net/11452/33908Çalışmada 29 yazar bulunmaktadır. Bu yazarlardan sadece Bursa Uludağ Üniversitesi mensuplarının girişleri yapılmıştır.Introduction: A nationwide multicentre study was conducted to establish well-defined reference intervals (Rls) of haematological parameters for the Turkish population in consideration of sources of variation in reference values (RVs). Materials and methods: K2-EDTA whole blood samples (total of 3363) were collected from 12 laboratories. Sera were also collected for measurements of iron, UIBC, TIBC, and ferritin for use in the latent abnormal values exclusion (LAVE) method. The blood samples were analysed within 2 hours in each laboratory using Cell Dyn and Ruby (Abbott), LH780 (Beckman Coulter), or XT-2000i (Sysmex). A panel of freshly prepared blood from 40 healthy volunteers was measured in common to assess any analyser-dependent bias in the measurements. The SD ratio (SDR) based on ANOVA was used to judge the need for partitioning RVs. Rls were computed by the parametric method with/without applying the LAVE method. Results: Analyser-dependent bias was found for basophils (Bas), MCHC, RDW and MPV from the panel test results and thus those Rls were derived for each manufacturer. Rls were determined from all volunteers' results for WBC, neutrophils, lymphocytes, monocytes, eosinophils, MCV, MCH and platelets. Gender-specific Rls were required for RBC, haemoglobin, haematocrit, iron, UIBC and ferritin. Region-specific Rls were required for RBC, haemoglobin, haematocrit, UIBC, and TIBC. Conclusions: With the novel use of a freshly prepared blood panel, manufacturer-specific Rls' were derived for Bas, Bas%, MCHC, RDW and MPV. Regional differences in Rls were observed among the 7 regions of Turkey, which may be attributed to nutritional or environmental factors, including altitude.eninfo:eu-repo/semantics/openAccessMedical laboratory technologyMulticentre studyReference intervalsComplete blood countHaematologyTurkeyWorldwide multicenterGlobal multicenterCell countsAnalytesAltitudeIndexesAdultAdolescentAdultAgedBlood cell countFemaleHematologic testsLaboratoriesHumansLaboratory proficiency testingMaleMiddle agedQuality controlReference valuesRegression analysisReproducibility of resultsTurkeyYoung adultArticle0004045057000132-s2.0-8502068912627235037728694726Medical laboratory technologyClinical Chemistry; Thyrotropin; Chemical SpeciesFerritinAdultAgedAnalytical parametersArticleBlood clottingBlood samplingFemaleHematological parametersHumanMaleMiddle agedMulticenter studyQuality controlQuestionnaireRed blood cell distribution widthReference valueAdolescentBlood cell countBlood examinationClinical trialDevicesGood laboratory practiceLaboratoryProceduresReference valueRegression analysisReproducibilityStandardsTurkeyYoung adult