2024-03-042024-03-042018-01-01Aybastıer, Ö. vd. (2018). ''Investigation of antioxidant ability of grape seeds extract to prevent oxidatively induced DNA damage by gas chromatography-tandem mass spectrometry''. Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences, 1072, 328-335.1570-02321476-5381https://www.sciencedirect.com/science/article/pii/S1570023217314770https://hdl.handle.net/11452/40181Phenolic compounds have been studied elaborately for their efficacy to improve health and to protect against a wide variety of diseases. Herein this study, different analysis methods were implemented to evaluate the antioxidant properties of catechin and cyanidin using their standard substances and as they found in the grape seeds extracts. Total phenol contents were 107.39 +/- 8.94 mg GAE/g dw of grape seeds for grape seed extract (GSE) and 218.32 +/- 10.66 mg GAE/g dw of grape seeds for acid-hydrolyzed grape seed extract (AcGSE). The extracts were analyzed by HPLC-DAD system and the results showed the presence of catechin, gallic acid, chlorogenic acid and ellagic acid in the processed methanolic extract and cyanidin, gallic acid and ellagic acid in the processed acidified methanolic extract. The protective abilities of catechin and cyanidin were tested against the oxidation of DNA. The results showed that cyanidin has better protection of DNA against oxidation than catechin. GSE and AcGSE were revealed to inhibit the oxidatively induced DNA damage. GSE decreased about 57% of damage caused by the Fenton control sample. This study could show new aspects of the antioxidant profiles of cyanidin and catechin.eninfo:eu-repo/semantics/closedAccessBiochemistry & molecular biologyChemistryDNA oxidationGrape seedCatechinCyanidinAntioxidantGC-MS/MSDNADrug productsEllagic acidFlavonoidsGas chromatographyMass spectrometryOrganic compoundsOxidationAntioxidant propertiesDNA oxidationGas chromatography-tandem mass spectrometryGrape seedsMethanolic extractsTotal phenol contentsPhenolsAnimalsAnthocyaninsAntioxidantsCatechinCattleDNADNA damageGas chromatography-mass spectrometryPhenolsPlant extractsSeedsTandem mass spectrometryVitisArticle0004335679000052-s2.0-85037333475328335107229223045https://doi.org/10.1016/j.jchromb.2017.11.044Biochemical research methodsChemistry, analyticalGrape Pomace; Antioxidant; VitisCatechinChlorogenic acidCyanidinEllagic acidFerrous gluconateGallic acidGrape seed extractUnclassified drugAnthocyaninAntioxidantCalf thymus DNACatechinCyanidinDNAPhenol derivativePlant extractAntioxidant activityArticleDNA damageDrug determinationDrug structureGrapeHigh performance liquid chromatographyMass fragmentographyOxidationOxidative stressPriority journalAnimalBovineChemistryDNA damageDrug effectsMass fragmentographyPlant seedProceduresTandem mass spectrometryVitis