Pehlivan, SultanAkyol, SümeyyaEren, FilizGüleç, Mehmet AkifEren, BülentDemircan, KadirAkyol, Ömer2023-04-062023-04-062016-08Pehlivan, S. vd. (2016). "The role of ADAMTS1 and versican in human myocardial infarction: A postmortem study". Laboratory Medicine, 47(3), 205-212.0007-50271943-7730https://doi.org/10.1093/labmed/lmw022https://academic.oup.com/labmed/article/47/3/205/2453835http://hdl.handle.net/11452/32234Objective: To determine the role of a disintegrin and metalloproteinase with thrombospondin type 1 motif (ADAMTS1) and fragmented versican in the myocardial infarction (MI) process in humans and to evaluate the diagnostic efficacy of ADAMTS1 for postmortem diagnosis of MI. Methods: Thirty autopsied individuals were allocated into 2 groups, namely, a study group of individuals who died of myocardial infarction (n = 20), and a control group who died of trauma (n = 10). We performed standard immunohistochemical staining on myocardial tissue specimens, studying anti-ADAMTS1, anti-versican, and anti-versican C terminal peptide sequence (DPEAAE) fragments. Results: Strong, diffuse staining was observed throughout myocardial tissue for ADAMTS1 in the 2 groups. However, in the study group, we observed no expression for ADAMTS1 around fibrotic areas but detected slight staining in coagulative and necrotic zones. Conclusion: Similar localizations of ADAMTS and fragmented versican in human heart tissue indicate that versican presumably is cleaved by ADAMTS1. Hence, ADAMTS1 can be regarded as a new marker for postmortem differential diagnosis of MI.eninfo:eu-repo/semantics/openAccessMedical laboratory technologyADAMTS1Myocardial infarctionVersicanFragmented versicanPostmortemDiagnosisSudden cardiac deathHeart-failureEndothelial-cellsStable anginaGeneExpressionFamilyAssociationMotifsADAMTS1 proteinAdolescentAdultAgedAged, 80 and overFemaleHumansImmunohistochemistryMaleMiddle agedMyocardial infarctionMyocardiumPathologyVersicansYoung adultArticle0003818798000072-s2.0-8501444025020521247327346868Medical laboratory technologyProtein; Disintegrins; AggrecanaseADAMTS1 proteinVersicanADAMTS1 proteinADAMTS1 protein, humanVCAN protein, humanVersicanAdipose tissueAdultAgedArticleAutopsyCarboxy terminal sequenceCell degenerationControlled studyCytolysisFemaleHeartHeart infarctionHeart interstitiumHeart muscle necrosisHumanHuman tissueImmunohistochemistryMaleMiddle agedMuscle cellMuscle fibrilMyocytolysisNerve fiberNeutrophil chemotaxisProtein expressionVacuolar degenerationVascular endotheliumVery elderlyAdolescentCardiac muscleEvaluation studyHeart infarctionPathologyProceduresYoung adult