Özel, Mustafa Zafer2023-10-042023-10-042015-05-01Arı, F. vd. (2015). "Promising anticancer activity of a lichen, Parmelia sulcata Taylor, against breast cancer cell lines and genotoxic effect on human lymphocytes". Cytotechnology, 67(3), 531-543.0920-9069https://doi.org/10.1007/s10616-014-9713-4https://link.springer.com/article/10.1007/s10616-014-9713-4http://hdl.handle.net/11452/34190Plants are still to be explored for new anticancer compounds because overall success in cancer treatment is still not satisfactory. As a new possible source for such compounds, the lichens are recently taking a great attention. We, therefore, explored both the genotoxic and anti-growth properties of lichen species Parmelia sulcata Taylor. The chemical composition of P. sulcata was analyzed with comprehensive gas chromatography-time of flight mass spectrometry. Anti-growth effect was tested in human breast cancer cell lines (MCF-7 and MDA-MB-231) by the MTT and ATP viability assays, while the genotoxic activity was studied by assays for micronucleus, chromosomal aberration and DNA fragmentation in human lymphocytes culture. Cell death modes (apoptosis/necrosis) were morphologically assessed. P. sulcata inhibited the growth in a dose-dependent manner up to a dose of 100 mu g/ml and induced caspase-independent apoptosis. It also showed genotoxic activity at doses (>125 mu g/ml) higher than that required for apoptosis. These results suggest that P. sulcata may induce caspase-independent apoptotic cell death at lower doses, while it may be genotoxic at relatively higher doses.eninfo:eu-repo/semantics/openAccessParmelia sulcataCell deathApoptosisBreast cancerTreatmentDNA damageIn-vitroCytotoxic activitiesAntioxidantMetabolitesExtractsAssayMurineBiotechnology & applied microbiologyCell biologyArticle0003516895000142-s2.0-8489659090953154367324676908Biotechnology & Applied microbiologyCell biologyUsnic acid; Extract; Lichen (disease)