Lichens exerts an anti-proliferative effect on human breast and lung cancer cells through induction of apoptosis

Erkısa, MerveUlukaya, Engin2024-01-162024-01-162021Öztürk, Ş. vd. (2021). "Lichens exerts an anti-proliferative effect on human breast and lung cancer cells through induction of apoptosis". Drug and Chemical Toxicology, 44(3), 259-267.0148-05451525-6014https://doi.org/10.1080/01480545.2019.1573825https://www.tandfonline.com/doi/full/10.1080/01480545.2019.1573825https://hdl.handle.net/11452/39058Successful cancer treatment still requires new complexes or compounds from natural sources. Therefore, we investigated anti-growth/apoptotic effects of methanol extracts of the lichen species (Xanthoparmelia somloensis (Gleyn.) Hale, Usnea intermedia (A. Massal.) Jatta, Bryoria capillaris (Ach.) Brodo & D. Hawksw and Lobaria pulmonaria (L.) Hoffm.) on human lung (A549, H1299) and breast (MCF-7, MDA-MB-231) cancer cell lines. Anti-growth effects were monitored by the MTT and ATP viability assays. Cell death mode was evaluated by employing the fluorescence staining of nucleus, caspase-cleaved cytokeratin 18 detection, caspase 3/7 activity assay, Anneksin V cytofluorimetric assay and mitochondria membrane potential assay. Among the lichen extracts, Usnea intermedia exhibited strong anti-growth activity in a dose-dependent manner (1.56-100 mu g/ml) compared to the others. Usnea intermedia was especially cytotoxic against MDA-MB-231 and H1299 cells (IC50 value for was found 3.0 and 10.2 mu g/ml respectively). The cytotoxicity was resulted from apoptosis as proved by the presence of pyknotic nuclei, caspase 3/7 activity, phosphatidylserine translocation and loss of mitochondria membrane potential. In conclusion, Usnea intermedia warrants for further in vivo evaluation as a new alternative in cancer treatment.eninfo:eu-repo/semantics/closedAccessApoptosisCancer cell deathCytotoxicityLichensUsnea intermediaA549 cellsAntineoplastic agents, phytogenicApoptosisBreast neoplasmsCell line, tumorCell proliferationDose-response relationship, drugFemaleHumansInhibitory concentration 50LichensLung neoplasmsMCF-7 cellsMembrane potential, mitochondrialPlant extractsLichens exerts an anti-proliferative effect on human breast and lung cancer cells through induction of apoptosisArticle0006275985000042-s2.0-8506256927925926744330835567Chemistry, MultidisciplinaryPharmacology & PharmacyToxicologyUsnic Acid; Extract; Lichen (Disease)Adenosine triphosphateAntineoplastic agentBryoria capillaris extractCaspase 3Caspase 7Cytokeratin 18Lichen extractLipocortin 5Lobaria pulmonaria extractMethanolPaclitaxelPhosphatidylserineUnclassified drugUsnea intermedia extractXanthoparmelia somloensis extractAntineoplastic agentPlant extractA-549 cell lineAntiproliferative activityArticleBreast cancerBryoria capillarisCancer cell lineCancer inhibitionCell deathCell nucleusCell viabilityConcentration responseControlled studyDrug cytotoxicityEnzyme activityFlow cytometryFluorescence imagingHumanHuman cellIC50In vivo studyLichen (organism)Lobaria pulmonariaLung cancerMCF-7 cell lineMDA-MB-231 cell lineMitochondrial membrane potentialMTT assayNCI-H1299 cell lineNonhumanParmeliaceaeProapoptotic activityProtein cleavageProtein transportUsneaUsnea intermediaXanthoparmelia somloensisApoptosisBreast tumorCell proliferationChemistryComparative studyDose responseDrug effectFemaleLung tumorTumor cell line