Harrison, William T. A.2022-11-112022-11-112013-02İçsel, C. vd. (2013). "Trans-Dichloridopalladium(II) and platinum(II) complexes with 2-(hydroxymethyl)pyridine and 2-(2-hydroxyethyl)pyridine: Synthesis, structural characterization, DNA binding and in vitro cytotoxicity studies". European Journal of Medicinal Chemistry, 60, 386-394 .0223-52341768-3254https://doi.org/10.1016/j.ejmech.2012.12.002https://www.sciencedirect.com/science/article/pii/S0223523412007246http://hdl.handle.net/11452/29450Four trans-palladium(II)- and trans-platinum(II)-chlorido complexes, trans-[PdCl2(2-hmpy)(2)] (1), trans-[PtCl2(2-hmpy)(2)] (2), trans-[Pdcl(2)(2-hepy)(2)] (3) and trans-[PtCl2(2-hepy)(2)] (4) (2-hmpy = 2-(hydroxymethyl)pyridine and 2-hepy = 2-(2-hydroxyethyl)pyridine), have been synthesized and characterized by elemental analysis, IR, NMR, and X-ray diffraction. The binding properties of these complexes with fish sperm DNA (FS-DNA) were investigated by UV titration, viscosity, thermal denaturation and electrophoresis measurements. The complexes can bind to FS-DNA and complex 4 exhibits the highest binding constant. Gel electrophoresis assay demonstrates that all the complexes can cleave the pCMV-beta gal plasmid DNA to a different degree. The cytotoxic activities of the complexes were tested against four different cancer cell lines. In general, the platinum(II) complexes are more effective than the isostructural palladium(II) complexes. Complex 4 shows high anticancer activity, compared to transplatin, cisplatin, carboplatin and oxaliplatin.eninfo:eu-repo/semantics/closedAccessPharmacology & pharmacyTransplatinum complexes2-(Hydroxymethyl)pyridine2-(2-Hydroxyethyl)pyridineDNA bindingCytotoxic activityTrans geometryEthidium-bromideMetal-complexesAntitumorPalladium(II)Pd(II)LigandDrugsRuthenium(II)FluorescenceAnimalsAntineoplastic agentsBinding sitesCell line, tumorCell proliferationCHO cellsCricetinaeDNADose-response relationship, drugDrug screening assays, antitumorHumansMolecular structureOrganoplatinum compoundsPalladiumPlatinumPyridinesRatsStructure-activity relationshipArticle0003162427000392-s2.0-848721604073863946023314052Chemistry, medicinalAntineoplastic Activity; Prodrugs; TransplatinCisplatinDNAPalladium complexPlasmid DNAPlatinum complexPyridine derivativeTransplatinAbsorption spectroscopyAnimal cellAntineoplastic activityArticleCancer cellCancer cell cultureCarbon nuclear magnetic resonanceCell viabilityColon cancerConcentration responseControlled studyCrystal structureCytotoxicityDNA bindingDNA denaturationDrug synthesisFibroblastGel electrophoresisHumanHuman cellIn vitro studyInfrared spectrophotometryInteractions with DNANonhumanRatStructure analysisTitrimetryUltraviolet radiationViscosityX ray diffraction