Taga, Yavuz2021-08-312021-08-312003-03İlçöl, Y.Ö. vd. (2003). “Choline increases serum insulin in rat when injected intraperitoneally and augments basal and stimulated aceylcholine release from the rat minced pancreas in vitro”. European Journal of Biochemistry, 270(5), 991-999.0014-2956https://doi.org/10.1046/j.1432-1033.2003.03472.xhttps://febs.onlinelibrary.wiley.com/doi/full/10.1046/j.1432-1033.2003.03472.xhttp://hdl.handle.net/11452/21571Intraperitoneal injection of choline (30-90 mg.kg(-1) ) produced a dose-dependent increase in serum insulin, glucose and choline levels in rats. The increase in serum insulin induced by choline (90 mg.kg(-1) ) was blocked by pretreatment with the muscarinic acetylcholine receptor antagonists, atropine (2 mg.kg(-1) ), pirenzepine (2 mg.kg(-1) ) and 4-diphenylacetoxy-N -methylpiperidine (2 mg.kg(-1) ) or the ganglionic nicotinic receptor antagonist, hexamethonium (15 mg.kg(-1) ). The effect of choline on serum insulin and glucose was enhanced by oral glucose administration (3 g.kg(-1) ). Choline administration was associated with a significant (P < 0.001) increase in the acetylcholine content of pancreatic tissue. Choline (10-130 mum) increased basal and stimulated acetylcholine release but failed to evoke insulin release from the minced pancreas at considerably higher concentrations (0.1-10 mm). Hemicholium-3, a choline uptake inhibitor, attenuated the increase in acetylcholine release induced by choline augmentation. Choline (1-32 mm) inhibited [(3) H]quinuclidinyl benzilate binding to the muscarinic receptors in the pancreatic homogenates. These data show that choline, a precursor of the neurotransmitter acetylcholine, increases serum insulin by indirectly stimulating peripheral acetylcholine receptors through the enhancement of acetylcholine synthesis and release.eninfo:eu-repo/semantics/openAccessBiochemistry & molecular biologyAnimaliaStaphylococcus phage 3APrecursorAcetylcholineParasympatheticMuscarinic reseptorsNicotinic receptorMuscarinic receptor subtypesIslet hormone-secretionAcetylcholine-releaseConscious ratsTyrosine-hydroxylaseGlucagon-secretionBrainCiticolineStriatumDiseaseAcetylcholineAnimalsBlood glucoseCholineGlucoseInjections, intraperitonealInsulinMaleMuscarinic antagonistsNicotinic antagonistsPancreasRatsRats, wistarArticle0001811074000222-s2.0-0037337246991999270512603332Biochemistry & molecular biologyCiticoline; Neuroprotective Agents; Glycerylphosphorylcholine4 diphenylacetoxy 1 methylpiperidineAcetylcholineAtropineCholineCholinergic receptorCholinergic receptor blocking agentGlucoseHemicholinium 3HexamethoniumİnsulinNicotinic receptor blocking agentPirenzepineQuinuclidinyl benzilateTritiumAcetylcholine releaseAnimal experimentAnimal tissueControlled studyDose responseDrug antagonismDrug effectDrug receptor bindingHomogenateHormone synthesisİnsulin blood levelİnsulin releaseNonhumanPancreasPriority journalRatStimulation