Buters, JeroenPrank, MarjeSofiev, MikhailPusch, GudrunAlbertini, RobertoAnnesi, Isabella MaesanoAntunes, CeliaBehrendt, HeidrunBerger, UweBrandao, RuiGalan, CarmenGrewling, LukaszJackowiak, BogdanKennedy, RoyRantio, Auli LehtimakiReese, GeraldSauliene, IngridaSmith, MattThibaudon, MichelWeber, BernhardCecchi, Lorenzo2022-05-112022-05-112015-07Buters, J. vd. (2009). "Variation of the group 5 grass pollen allergen content of airborne pollen in relation to geographic location and time in season the HIALINE working group". Journal of Allergy and Clinical Immunology, 136(1), 87-U179.0091-6749https://doi.org/10.1016/j.jaci.2015.01.049https://www.sciencedirect.com/science/article/pii/S0091674915004121http://hdl.handle.net/11452/26387Background: Allergies to grass pollen are the number one cause of outdoor hay fever. The human immune system reacts with symptoms to allergen from pollen. Objective: We investigated the natural variability in release of the major group 5 allergen from grass pollen across Europe. Methods: Airborne pollen and allergens were simultaneously collected daily with a volumetric spore trap and a high-volume cascade impactor at 10 sites across Europe for 3 consecutive years. Group 5 allergen levels were determined with a Phl p 5-specific ELISA in 2 fractions of ambient air: particulate matter of greater than 10 mu m in diameter and particulate matter greater than 2.5 mu m and less than 10 mu m in diameter. Mediator release by ambient air was determined in Fc epsilon RI-humanized basophils. The origin of pollen was modeled and condensed to pollen potency maps. Results: On average, grass pollen released 2.3 pg of Phl p 5 per pollen. Allergen release per pollen (potency) varied substantially, ranging from less than 1 to 9 pg of Phl p 5 per pollen (5% to 95% percentile). The main variation was locally day to day. Average potency maps across Europe varied between years. Mediator release from basophilic granulocytes correlated better with allergen levels per cubic meter (r(2) = 0.80, P < .001) than with pollen grains per cubic meter (r(2) = 0.61, P < .001). In addition, pollen released different amounts of allergen in the non-pollenbearing fraction of ambient air, depending on humidity. Conclusion: Across Europe, the same amount of pollen released substantially different amounts of group 5 grass pollen allergen. This variation in allergen release is in addition to variations in pollen counts. Molecular aerobiology (ie, determining allergen in ambient air) might be a valuable addition to pollen counting.eninfo:eu-repo/semantics/closedAccessAllergyImmunologyAllergenAllergyBasophilEuropeExposureGrassHIALINEModelingPhl p 5PM10PM2.5SILAMThunderstorm-related asthmaBet v 1Birch pollenCreate projectTimothy grassHay-feverReleaseIgeStandardizationSensitizationAirBasophilsCell degranulationEuropeHumansHumidityParticulate matterPlant proteinsPoaceaePollenReceptors, IgERhinitis, allergic, seasonalSeasonsArticle0003575422000102-s2.0-8493719115887U179136125956508AllergyImmunologyRagweed; Ambrosia Artemisiifolia; PollenAllergenFc receptorFcepsilonRI gamma-chain, humanImmunoglobulin E receptorParticulate matterPhl p V protein, Phleum pratenseVegetable proteinAmbient airArticleBasophilEnzyme linked immunosorbent assayEuropeGeographic distributionGrass pollenHumanHumidityMediator releaseParticulate matterPollen allergyPriority journalSeasonal variationVolumetryAirAnalysisChemistryDegranulationGeneticsImmunologyMetabolismPhysiologyPoaceaePollenRhinitis, allergic, seasonalSeason