Kocyiğit, AbdurrahimGüler, Eray MetinDurmuş, EzgiYenigün, Vildan BetülKanımdan, EbruÖzman, ZeynepYaşar, ÖznurGören, Ahmet CeyhanHekimoğlu, Emine RumeysaOruç, Hasan HüseyinAydoğdu, Gökhan2024-11-272024-11-272023-08-081612-1872https://doi.org/10.1002/cbdv.202300591https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202300591https://hdl.handle.net/11452/48532In this study, we investigated the combined treatment of 5-fluorouracil (5-FU) and Anatolian propolis extract (PE) on colorectal cancer (CRC)using in vitro and in vivo studies. We exposed luciferase-transfected (Lovo-Luc CRC) cells and healthy colon cells (CCD-18Co) to varying concentrations of 5-FU and PE to assess their genotoxic, apoptotic, and cytotoxic effects, as well as their intracellular reactive oxygen species (iROS) levels. We also developed a xenograft model in nude mice and evaluated the anti-tumor effects of PE and 5-FU using various methods. Our findings showed that the combination of PE and 5-FU had selectivity against cancer cells, particularly at higher doses, and enhanced the anti-tumor effectiveness of 5-FU against colon CRC. The results suggest that PE can reduce side effects and increase the effectiveness of 5-FU through iROS generation in a dose-dependent manner.eninfo:eu-repo/semantics/closedAccessColon-cancerBiological-propertiesInduced apoptosisBreast-cancerCell-lineTumorChemotherapyFlavonoidsCisplatinLiverPropolisColorectal cancer5-fluorouracilIn vivo animal imaging systemXenograft modelsBiochemistry & molecular biologyChemistryPropolis enhances 5-fluorouracil mediated antitumor efficacy and reduces side effects in colorectal cancer: An in Vitro and in Vivo studyArticle00104389960000120910.1002/cbdv.202300591