2022-10-072022-10-072011Cengiz, M. vd. (2011). "In vitro phannacodynaniics of enrofloxacin against an Escherichia coli gyrA mutant". Journal of Animal and Veterinary Advances, 10(18), 2384-2388.1680-5593https://doi.org/10.3923/javaa.2011.2384.2388https://medwelljournals.com/abstract/?doi=javaa.2011.2384.2388http://hdl.handle.net/11452/29030The aim of this study was to investigate in vitro the pharmacodynamics of Enrofloxacin (ENR) against an Escherichia coli gyrA mutant (E. coli MT/128). Broth microdilution testing was used to determine the Minimum Inhibitory Concentration (MIC) and multi-step resistance selection was performed until reaching 1 mu g ML(-1) MIC. For time-kill experiments, colony counts were determined by plating each diluted sample onto Plate Count Agar and an integrated pharmacokinetic/pharmacodynamics area measure (log ratio area) was applied to all cfu data. A single C-T mutation was found in gyrA at codon 83. Concentration-dependent bacterial killing was observed for E. coli MT/128. Bactericidal activity for this strain was achieved within 4 h at concentrations >= 8 times the MIC with no significant regrowth by 24 h.eninfo:eu-repo/semantics/closedAccessVeterinary sciencesPharmacodynamic of enrofloxacinEscherichia coliC-T mutationSignificantMicrodilutionTurkeyColony-variant phenotypeFluoroquinolone resistanceStaphylococcus-aureusStreptococcus-pneumoniaeQuinolone resistanceMechanismsMutationsSelectionAnimalsModelArticle0002980945000092-s2.0-84872149544238423881018Veterinary sciencesEscherichia Coli; Plasmids; Enterobacteriaceae