2022-09-092022-09-092015-02-10Ertürk, E. vd. (2015). "BRCA mutations cause reduction in miR-200c expression in triple negative breast cancer". Gene, 556(2), 163-169.0378-11191879-0038https://doi.org/10.1016/j.gene.2014.11.047https://www.sciencedirect.com/science/article/pii/S0378111914013249http://hdl.handle.net/11452/28614Triple negative breast cancer (TNBC) is the most aggressive and poorly understood subclass of breast cancer (BC). Over the recent years, miRNA expression studies have been providing certain detailed overview that aberrant expression of miRNAs is associated with TNBC. Although TNBC tumors are strongly connected with loss of function of BRCA genes, there is no knowledge about the effect of BRCA mutation status on miRNA expressions in TNBC cases. The aims of this study were to evaluate the expression profile of miRNAs that plays role in TNBC progression and the role of BRCA mutations in their regulation. The expression level of BC associated 13 miRNAs was analyzed in 7 BRCA mutations positive, 6 BRCA mutations negative TNBC cases and 20 non-tumoral tissues using RT-PCR. According to RT2 Profiler PCR Array Data Analysis, let-7a expression was 4.67 fold reduced in TNBCs as compared to normal tissues (P = 0.031). In addition, miR-200c expression was 5.75 fold reduced in BRCA mutation positive TNBC tumors (P = 0.005). Analysis revealed a negative correlation between miR-200c and VEGFA expressions (r = -468). Thus, miR-200c may be involved in invasion and metastasis in TNBC cases with BRCA mutation. In this study we provide the knowledge on the first report of association between microRNA-200c and BRCA mutations in TNBC. Further studies and evaluations are required, but this miRNA may provide novel therapeutic molecular targets for TNBC treatment and new directions for the development of anticancer drugs.eninfo:eu-repo/semantics/closedAccessBRCA1/2 gene mutationsMicroRNAMiR-200cTriple negative breast cancerEpithelial-mesenchymal transitionDown-regulationOvarian-cancerFeedback loopFamilyZeb1MicroRNA-200cProgressionMetastasisResistanceAdultBRCA1 proteinBRCA2 proteinDisease progressionFemaleGene expression regulationNeoplasticHumansMicroRNAsMiddle agedMutationTriple negative breast neoplasmsVascular endothelial growth factor AArticle0003495898000122-s2.0-84919872180163169556225445393Genetics & HeredityCirculating Microrna; Stomach Neoplasms; Breast NeoplasmsBRCA1 proteinBRCA1 protein, humanBRCA2 proteinBRCA2 protein, humanMicroRNAMırn200 microrna, humanMirnlet7 microRNA, humanVasculotropin AVEGFA protein, humanAdultArticleCancer growthClinical articleControlled studyFemaleGene expression profilingGene expression regulationGene mutationHumanHuman tissueLet 7a geneOncogeneReverse transcription polymerase chain reactionTriple negative breast cancerTumor invasionVEGFA geneDisease courseGeneticsMiddle agedMutationPathologyTriple negative breast cancer