Solak, BernaKarkucak, MutluTuran, HakanUslu, EsmaErdem, Teoman2022-12-202022-12-202015-11-03Solak, B. vd. (2016). "Glutathione S-transferase M1 and T1 gene polymorphisms in patients with chronic plaque-type psoriasis: A case-control study". Medical Principles and Practice, 25(2), 155-158.1011-75711423-0151https://doi.org/10.1159/000442165https://www.karger.com/Article/FullText/442165https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588339/http://hdl.handle.net/11452/29990Objective: To determine the role of glutathione S-transferase (GST) isoenzyme polymorphisms as susceptibility factors in patients with psoriasis in a Turkish cohort. Subjects and Methods: In this case-control study, 105 patients with plaque-type psoriasis and 102 healthy controls were recruited from the dermatology outpatient clinics of two university hospitals. Genomic DNA was extracted from whole blood using a DZ DNA isolation kit. Multiplex PCR was used to determine GSTM1 and GSTT1 polymorphisms in the isolated DNAs. Results: Of the 150 patients with psoriasis, 83 (79%) were identified with the GSTT1 genotype and 22 (21%) with the null genotype. Of the 102 patients in the control group, 69 (67.6%) subjects were identified with the GSTT1 genotype and 33 (32.4%) with the null genotype. There was no significant difference between the patient and control groups (p = 0.063). Regarding the GSTM1 polymorphism, 54 (51.4%) patients were identified with this genotype and 51 (48.6%) with the null genotype; in the control group, 50 (49%) were identified with this genotype and 52 (51%) with the null genotype. Again there was no statistically significant difference between the groups (p = 0.957). Conclusion: In this Turkish cohort of patients with psoriasis, neither GSTT1 nor GSTM1 polymorphisms were associated with disease susceptibility. Larger studies with a wider range of GST isoenzyme are needed.eninfo:eu-repo/semantics/openAccessGeneral & internal medicineGene polymorphismsGlutathione S-transferasePsoriasisCancer riskSusceptibilityAssociationPopulationGenotypeSensitivitySkinCase-control studiesFemaleGenotypeGlutathione transferaseHumansMalePolymerase chain reactionPolymorphism, geneticPsoriasisRisk factorsTurkeyArticle0003700810000092-s2.0-8495810507115515825226535568Medicine, general & internalDimethyl Fumarate; Fumaric Acid; Multiple SclerosisGenomic DNAGlutathione transferase M1Glutathione transferase T1Glutathione S-transferase M1Glutathione S-transferase T1Glutathione transferaseAdultAgedArticleCase control studyControlled studyDNA isolationDNA polymorphismFemaleGenetic associationGenetic susceptibilityGenotypeHumanMajor clinical studyMalePsoriasis vulgarisGenetic polymorphismGeneticsPolymerase chain reactionPsoriasisTurkeyRisk factor