Shahzad, A. H.Sattar, A.Ahmad, N.Ahmad, IYousaf, M. S.Abbas, S.Blouch, R. S.Nak, D.Nak, Y.Ray, D. L.Silvia, W. J.2024-11-182024-11-182020-01-010375-1589https://doi.org/10.4314/sajas.v50i6.4https://www.ajol.info/index.php/sajas/article/view/205174https://hdl.handle.net/11452/48004The studies aimed to evaluate the pregnancy rate (PR) for timed artificial insemination (TAI) after G7G-Ovsynch, modified G7G-Ovsynch (MG7G-Ovsynch) and Ovsynch protocols and to assess the accuracy of using pregnancy-associated glycoproteins (PAGs) and plasma progesterone (P-4) in pregnancy diagnosis compared with ultrasonography (US). In study 1, Holstein cows (n = 37) were bred by TAI following the G7G-Ovsynch protocol (n = 19) or MG7G-Ovsynch (n = 18). Pregnancy was evaluated by US at days 31, 59, and 87 after breeding. The PR was not different for the G7G-Ovsynch and MG7G-Ovsynch. Blood and milk samples were collected on day 3 after insemination and then weekly through day 59 post TAI in cows diagnosed as not pregnant on day 31 and through day 87 in pregnant cows. PAGs were measured using ELISA and P-4 by radioimmunoassay (RIA). In the second study, Holstein cows (n = 212) were bred by TAI following G7G-Ovsynch protocol (n = 110) or standard Ovsynch (n = 102). Cows were subjected to pregnancy diagnosis on days 30, 60, and 90. A subset (n = 15 in each group) was subjected to blood and milk samples on days 30, 45, 60, 75, and 90 to measure PAGs and P-4. In study 2, PR was not significantly different between synchronization protocols on days 30, 60, and 90. Pregnancy loss averaged 15% between day 30 and day 90. The use of PAGs and P-4 proved equally effective in diagnosis of pregnancy. Thus, G7G-Ovsynch was deemed the protocol of choice in postpartum cows, and PAGs assayed in milk or plasma could be used to diagnose pregnancy.eninfo:eu-repo/semantics/openAccessOvulatory follicleEmbryonic lossEstrous-cycleCowsPgf(2-alpha)ProgesteroneGlycoproteinPlasmaBeefInjectionArtificial inseminationOestrous synchronizationOvsynchPostpartum cowPregnancy associated glycoproteinsUltrasonographyAgricultureArticle00062152080000479379950610.4314/sajas.v50i6.4