2024-02-062024-02-062020-05Alçay, S. vd. (2020). "Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin-based extenders". Andrologia, 52(4).0303-45691439-0272https://onlinelibrary.wiley.com/doi/10.1111/and.13555https://hdl.handle.net/11452/39551The objective of this study was to determine the optimum concentrations of rainbow trout seminal plasma (RTS) supplemented extenders for goat semen quality at post-thaw and after incubation. Five sexually mature Saanen goat (Capra aegagrus hircus) were used for semen collection. Pooled semen was diluted with soybean lecithin-based extender without RTS (control) or supplemented with different concentrations of RTS (1%, 2%, 4% or 8%), at a final concentration of 150 x 10(6) spermatozoon/ml. Sperm motility, plasma membrane functional integrity (HOST), damaged acrosome (PSA-FITC), mitochondrial activity (rhodamine123) and DNA integrity (TUNEL) were evaluated. Spermatological parameters were evaluated at post-thaw and after 6 hr incubation. RTS8 group preserved sperm motility, acrosomal integrity, plasma membrane functional integrity and mitochondrial function better than the control group (p < .05). The study demonstrated that RTS supplemented lecithin-based extenders have useful effects on goat spermatozoa. In addition, the results of the current study represented the positive effect of using 8% RTS supplemented extender.eninfo:eu-repo/semantics/closedAccessPost-thaw incubationSaanen goatSeminal plasmaSperm freezingSoybean lecithinRam semenEgg-yolkIncubation resiliencePostthaw qualityLiquid storageSpermatozoaAntioxidantParametersEndocrinology & metabolismAnimalsCryopreservationDNA fragmentationGoatsLecithinsMaleOncorhynchus mykissSemenSperm motilityArticle0005167676000012-s2.0-8508011708852432107791https://doi.org/10.1111/and.13555AndrologyExtender; Semen; Semen ExtendersPhosphatidylcholineAcrosomeSeminal plasmaArticleCell membraneControlled studyCryopreservationDNA fragmentationFreeze thawingMaleMitochondrionNonhumanOncorhynchus mykissSaanen goatSoybeanSpermSperm qualitySpermatozoonSpermatozoon motilityAnimalGoat