2022-03-142022-03-142002Egel, Ç. vd. (2002). "Inhibitory effects of ascorbic acid and folinic acid on chromosome aberrations induced by pyrimethamine in vitro". Teratogenesis Carcinogenesis and Mutagenesis, 22(5), 353-362.02703211https://doi.org/10.1002/tcm.10030https://onlinelibrary.wiley.com/doi/10.1002/tcm.10030http://hdl.handle.net/11452/24982In this study, the anticlastogenic effects of ascorbic acid and the protective effect of folinic acid against the formation of chromosomal aberrations in humans by pyrimethamine were investigated. Pyrimethamine is a folic acid antagonist used for the treatment of malaria and toxoplasmosis. In this study, 18 different healthy people, who do not drink alcohol and are non-smokers, were chosen as an experimental group; 0.025 mg/ml pyrimethamine was given to the lymphocyte culture, which had been prepared with the peripheral blood taken from this group. After that each of the following doses were given to the same culture: 20, 40, and 80 mM of ascorbic acid and 25, 50, and 100 mM of folinic acid. The results of the cytogenetic evaluation showed that the aberrations due to pyrimethamine in the chromosomes were reduced by ascorbic acid and folinic acid significantly, depending on the given dose.eninfo:eu-repo/semantics/closedAccessAnticlastogenAscorbic acidChromosomal aberrationFolinic acidProtective effectPyrimethamineHuman-lymphocyte-culturesSister-chromatid exchangesVitamin-CFragile sitesV79 CellsIn-vitroGenotoxicityInductionDamageAgentsDose-response relationship, drugAscorbic acidCells, culturedChromosome aberrationsHumanDose-response relationshipFolic acid antagonistsLeucovorinMitosisPyrimethamineCells, culturedDrugHumansMitosisArticle0001777791000052-s2.0-003602518835336222512210498Oncology genetics & heredityToxicologyHyperpigmentation; Vitamin B 12 Deficiency; Megaloblastic AnemiaAscorbic acidClastogenChromosome aberrationPyrimethamineFolic acid antagonistFolinic acidToxoplasmosisAscorbic acidFolic acid antagonistPriority journalSmokingControlled studyAdultAlcohol consumptionArticleCell protectionChromosome aberrationCytogeneticsDose time effect relationLymphocyte cultureDose calculationDose responseEvaluationHuman experimentFemaleHumanHuman cellMalariaMaleNormal humanCell cultureDrug effectMitosis