Çetin, EceAta, Zafer2023-11-092023-11-092018-04Yıbar, A. vd. (2018). ''Clostridium perfringens contamination in retail meat and meat-based products in Bursa, Turkey''. Foodborne Pathogens and Disease, 15(4), 239-245.1535-31411556-7125https://doi.org/10.1089/fpd.2017.2350https://www.liebertpub.com/doi/10.1089/fpd.2017.2350http://hdl.handle.net/11452/34821This study examined the incidence of Clostridium perfringens in raw, ready-to-cook (RTC), and ready-to-eat (RTE) meat and meat-based products (N=306) collected from restaurants, supermarkets, and butcher shops in Bursa, Turkey. In addition, we investigated the presence of the C. perfringens enterotoxin (CPE), as well as cpe genes and their source (chromosomal or plasmid borne). In this study, tryptose sulfite cycloserine (TSC) agar for classic culture isolation and API and real-time polymerase chain reaction (RT-PCR) techniques were used to identify C. perfringens and detect cpa and cpe genes from these products, respectively. Seventeen C. perfringens isolates (5.6%) were isolated and identified with API 20A. In addition, 42 of 81 suspicious isolates (51.9%) were identified as C. perfringens using RT-PCR. Of the 81 suspicious isolates tested by RT-PCR, 22 (27.2%) carried the cpe gene either on the plasmid or chromosome. Twenty-one isolates were positive for chromosomal cpe (C-cpe), and one was positive for plasmid-borne cpe (P-cpe). CPE was detected in 31.8% (7/22) of the cpe positive isolates by the PET-RPLA test. In conclusion, C. perfringens and their CPEs were present in raw, RTC, and RTE meat and meat-based foods in this study. It is emphasized that the presence of C. perfringens and the cpe gene in these foods may be a potential risk for human health.eninfo:eu-repo/semantics/closedAccessFood science & technologyClostridium perfringensMeatMeat-based productsEnterotoxinEnterotoxin genesEnterotoxinChickenCpePrevalenceAnimalsHumansFoodsKebabBacterial proteinsClostridium perfringensEnterotoxinsFood contaminationFood microbiologyMeatMeat productsPlasmidsPolymerase chain reactionTurkeyArticle0004195585000012-s2.0-8504548166523924515429315008Food science & technologyNecrotizing Enteritis; Clostridium Perfringens; ToxinBacterial proteinEnterotoxinArticleBacterial geneBacterium contaminationBacterium cultureBacterium isolationClostridium perfringensControlled studyCpa geneDNA extractionMeat industryNonhumanPrevalencePriority journalReal time polymerase chain reactionReverse transcription polymerase chain reactionSmooth muscle contractionSynovial BursaAnalysisClostridium perfringensFood contaminationFood controlGeneticsIsolation and purificationMeatMicrobiologyPlasmidPolymerase chain reactionTurkey (bird)