İkitimur, Elif I. ArmutakGürel, Ebru GürevinYaylım, İlhanİşbilen, Banu BaşokŞennazlı, GülbinYüzbaşıoğlu, Gülay ÖztürkSönmez, KıvılcımÇelik, FarukKüçükhüseyin, ÖzlemKorkmaz, GurbetZeybek, Şakir Ümit2022-12-022022-12-022016-04-28İkitimur, A. E. I. vd. (2016). "Apoptosis-inducing effect of a palladium(II) complex-[PdCl(terpy)](sac)center dot 2H2O] on ehrlich ascites carcinoma (EAC) in mice". In Vivo, 30(4), 457-464.0258-851X1791-7549https://iv.iiarjournals.org/content/30/4/457.shorthttp://hdl.handle.net/11452/29655Background/Aim: New compounds for cancer treatment are needed due to persistenly unsatisfactory management of cancer. [PdCl(terpy)](sac)center dot 2H2O] (sac= saccharinate, and terpy= 2,2':6',2 ''- terpyridine) is a compound synthesized for this purpose. We investigated its anti-proliferative and pro-apoptotic effects on Ehrlich Ascites Carcinoma (EAC) in vivo. Materials and Methods: 42 Balb-c female mice were subcutaneously (s.c.) injected with EAC cells (1st day) and then randomly divided into 5 groups: control (0.9% NaCl), complex (2 mg/kg), complex (3 mg/kg) cisplatin (4 mg/kg) and paclitaxel (12.5 mg/kg). On the 5th and 12th day animals were drug administrated. At 14th day, animals were sacrificed. Expression of cell death and/or cell cycle-related markers (Bcl-2, Bax, active caspase-3, p53, PCNA) and apoptosis were investigated immunohisto-chemically. Survival-related markers (Akt, GSK-3 beta, IGF-1R, IR, IRS-1, p70S6K, PRAS40) were evaluated by luminex analysis. Results: Expression of p53, PCNA, Bcl-2 was found decreased (p< 0.001) and that of active caspase-3, Bax, and apoptotic cells was found increased (p< 0.001) in all groups. The survivalrelated markers did not show any statistical difference in complex groups. Conclusion: The Pd(II)-complex seems to have a strong anticancer activity on EAC by inducing apoptosis via both suppression of proliferation and activation of apoptosis in vivo, similar to the effects of cisplatin and paclitaxel.eninfo:eu-repo/semantics/closedAccessResearch & experimental medicinePd(II) complexEACBreast cancerApoptosisIn vivoBreast-cancer cellsNuclear antigen immunohistochemistryGene bax-alphaInsulin-receptor substrate-1Saccharinate complexIn-vitroTumor-growthDeathPhosphorylationTerpyridineAnimalsAntineoplastic agentsApoptosisApoptosis inducing factorCarcinoma, ehrlich tumorCell proliferationCisplatinCoordination complexesDrug therapy, combinationFemaleImmunoenzyme techniquesMiceMice, inbred BALB CPalladiumArticle0003794869000162-s2.0-8500818816245746430427381609Medicine, research & experimentalComplex; Palladium; 2-PhenylpyridineAntineoplastic agentApoptosis inducing factorCisplatinCoordination compoundPalladiumAnimalApoptosisBagg albino mouseCarcinoma, ehrlich tumorCell proliferationChemistryCombination drug therapyDrug effectsEnzyme immunoassayFemaleMetabolismMousePathology