2022-01-182022-01-182009-01-08İşbil, N. B. vd. (2009). "Peripheral GLP-1 gastroprotection against ethanol: The role of exendin, NO, CGRP, prostaglandins and blood flow". Regulatory Peptides, 152(1-3), 22-27.0167-0115https://doi.org/10.1016/j.regpep.2008.09.006https://www.sciencedirect.com/science/article/pii/S016701150800164Xhttp://hdl.handle.net/11452/24138The aim of this study was to investigate the effects of peripherally injected glucagon like peptide-1 (GLP-1) on ethanol-induced gastric mucosal damage and the mechanisms included in the effect. Absolute ethanol was administered through an orogastric cannula right after the injection of GLP-1 (1, 10, 100, 1000 or 10,000 ng/kg; i.p.). The rats were decapitated an hour later, the stomachs removed and the gastric mucosal damage scored. 1000 ng GLP-1 inhibited gastric mucosal damage by 45% and 10,000 ng GLP-1 by 60%. The specific receptor antagonist exendin-(9-39) (2500 ng/kg; i.p.), calcitonin gene related peptide (CGRP) receptor antagonist CGRP-(8-37) (10 pg/kg; i.p.), nitric oxide (NO) synthase inhibitor L-NAME (30 mg/kg; s.c) and cyclooxygenase inhibitor indomethacin (5 mg/kg; i.p.) inhibited the preventive effect of GLP-1 on ethanol-induced gastric mucosal damage. GLP-1 also prevented the decrease in gastric mucosal blood flow caused by ethanol when administered at gastroprotective doses (1000 and 10,000 ng/kg: i.p.). In conclusion, GLP-1 administered peripherally prevents the gastric mucosal damage caused by ethanol in rats. CGRR NO, prostaglandin and gastric mucosal blood flow are thought to play a role in this effect, mediated through receptors specific to GLP-1.eninfo:eu-repo/semantics/closedAccessCGRP-(8-37)Exendin-(9-39)Gastric mucosal blood flowGastric mucosal damageGlucagon like peptide-1Indomethacinl-NAMEGlucagon-like peptide-1Gene-related peptideGastric somatostatinExperimental-modelsRatReceptorLesionsAmylinAmideMechanismsEndocrinology & metabolismPhysiologyRattusAnimalsCalcitonin gene-related peptideEthanolGastric mucosaGlucagon-like peptide 1IndomethacinMaleNG-nitroarginine methyl esterNitric oxidePeptide fragmentsProstaglandinsRatsRats, sprague-dawleyRegional blood flowArticle0002627485000042-s2.0-5724909711622271521-318926859Endocrinology & metabolismPhysiologyGlucagon-Like Peptide-1 Receptor; Gastric Inhibitory Polypeptide; Exendin (9-39)AlcoholCalcitonin gene related peptide[8-37]Exendin 4Exendin[9-39]Glucagon like peptide 1Glucagon like peptide 1 receptorIndometacinN(g) nitroarginine methyl esterNitric oxideProstaglandin derivativeUnclassified drugAnimal experimentAnimal modelArticleControlled studyDrug effectMaleNonhumanPeptide analysisPriority journalProtein functionRatStomach blood flowStomach mucosa lesionStomach protection