2021-09-012021-09-012005Kocaeli, H. vd. (2005). "MK-801 improves neurological and histological outcomes after spinal cord ischemia induced by transient aortic cross-clipping in rats". Surgical Neurology, 64(Supplement 2), 22-27.0090-3019https://doi.org/10.1016/j.surneu.2005.07.034https://www.sciencedirect.com/science/article/pii/S009030190500532Xhttp://hdl.handle.net/11452/21620Background: Glutamergic excitotoxicity has been shown to play a deleterious role in the pathophysiology of ischemic spinal cord injury (ISCI). The aim of this study was to investigate the neuroprotective effect of a single dose of MK-801, an antiexcitotoxic drug, in a rat model of ISCI. Methods: Ischemic spinal cord injury was induced for 17 minutes in Sprague-Dawley rats using direct aortic arch, just proximal to the left common carotid artery, plus left subclavian artery cross-clamping through a left-sided limited thoracotomy. Study groups were as follows: control group (n = 8) receiving only vehicle and experimental group (n = 8) receiving a single dose of MK-801 (1 mg/kg IV) 10 minutes before aortic clamping. Neurological examination was performed at 6 hours, 24 hours, and daily up to 96 hours. Rats were sacrificed at 96 hours, and spinal cords were removed for histopathology. Results: All the control rats had severe permanent neurological deficits after ISCI, whereas the MK-801-treated rats had statistically (P <.05) better neurological outcome and good recovery. Histopathology revealed severe neuronal necrosis in the lumbar gray matter of control rats, whereas MK-801-treated rats showed mild injury. Conclusion: These results demonstrate that combined temporary clipping of the aortic arch (just proximal to the left common carotid artery) plus left subclavian artery for 17 minutes reproduces reliable paraplegia, and a single dose of MK-801 given before ISCI provides significant neuroprotection.eninfo:eu-repo/semantics/closedAccessAortaMK-801NeuroprotectionRatSpinal cord ischemiaThoracotomyGlutamate antagonistApoptosisModelInjuryParaplegiaPreventionDeficitsNeurosciences & neurologySurgeryArticle0002333210000062-s2.0-27444443134222764Supplement 2Clinical neurologySurgeryThoracic Aorta Aneurysm; Spinal Cord Ischemia; Endoleak