Acar, Hasan2022-04-212022-04-212003-09Yakut, T. vd. (2003). “Frequency of recombinant and nonrecombinant products of pericentric inversion of chromosome 1 in sperm nuclei of carrier: By FISH technique”. Molecular Reproduction and Development, 66(1), 67-71.1040-452Xhttps://doi.org/10.1002/mrd.10325https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.10325http://hdl.handle.net/11452/25931Meiotic segregation products of carriers with pericentric inversion are very important for assessing the risk of unbalanced forms and appropriate genetic counseling. We investigated the incidence of recombinant and nonrecombinant products of chromosome 1 with pericentric inversion, in the sperm nuclei of the carrier by using triple color fluorescence in situ hybridization (FISH). The centromere specific and telomere specific probes for chromosome 1 were used. In the segregation analysis, 1,636 sperm nuclei were analyzed; 82.5% of the sperms were including normal or inverted chromosome 1, and the dup(p)/del(q) and del(p)/dup(q) recombinant products in sperm nuclei of our carrier were 8.7 and 7.3%, respectively. The number of recombinant products may be dependent on the formation of an inversion loop, which the number of the formation of chiasmata results in the different number of normal/balanced and recombinant products. The use of FISH, using different probe combination, in sperm nuclei has proved to be an accurate approach to determine the meiotic segregation patterns and could help to better establish a reproductive prognosis and genetic counseling.eninfo:eu-repo/semantics/closedAccessBiochemistry and molecular biologyCell biologyDevelopmental biologyReproductive biologyCell nucleusChromosome aberrationsChromosomes, human, pair 1HumansIn situ hybridization, fluorescenceMaleSpermatozoaArticle0001845297000102-s2.0-0042267426677166112874801Biochemistry and molecular biologyCell biologyDevelopmental biologyReproductive biologyRobertsonian Chromosome Translocation; Balanced; Chromosome AberrationsArticleCell nucleusCentromereChromosome 1Chromosome chiasmChromosome segregationControlled studyFemaleFluorescence in situ hybridizationGenetic counselingHumanHuman cellMaleMeiosisNormal humanPericentric chromosome inversionPriority journalSpermTelomere