2022-06-152022-06-152014-10Ertürk, E. vd. (2014). "Expression status of let-7a and miR-335 among breast tumors in patients with and without germ-line BRCA mutations". Molecular and Cellular Biochemistry, 395(1-2), 77-88.0300-81771573-4919https://doi.org/10.1007/s11010-014-2113-4https://link.springer.com/article/10.1007/s11010-014-2113-4http://hdl.handle.net/11452/27180The genetic factors of cancer predisposition remain elusive in the majority of familial and/or early-onset cases of breast cancer (BC). This type of BC is promoted by germ-line mutations that inactivate BRCA1 or BRCA2. On the other hand, recent studies have indicated that alterations in the levels of miRNA expression are linked to this disease. Although BRCA1 and BRCA2 gene mutations have been reported to commonly lead to alterations in genes that encode cancer-related proteins, little is known regarding the putative impact of these mutations on noncoding miRNAs. In the present study, we aimed to determine whether miRNA dysregulation is involved in the pathogenesis of BRCA-mutated BC. An expression analysis of 14 human miRNAs previously shown to be related to BC diagnosis, prognosis, and drug resistance was conducted using tissues from 60 familial and/or early-onset patients whose peripheral blood samples had been screened for BRCA1 and BRCA2 mutations through sequence analysis. Let-7a and miR-335 expression levels were significantly downregulated in the tumors of patients with a BRCA mutation compared with those of patients without a BRCA mutation (P = 0.04 and P = 0.02, respectively). Our results defined the associations between the expression status of let-7a and miR-335 and BRCA mutations. The expression analysis of these miRNAs might be used as biomarkers of the BRCA mutation status of early-onset and/or familial BC.eninfo:eu-repo/semantics/closedAccessBreast cancerMicroRNABRCA1/2 gene mutationsLet-7aMiR-335MicroRNA expressionGene-expressionOvarian-cancerTurkish womenDiiferentiationProliferationResistancePrognosisSignatureVariantsCell biologyAdultAgedBRCA1 proteinBRCA2 proteinBreast neoplasmsDown-regulationFemaleGene expression regulation, neoplasticGerm-line mutationHumansMicroRNAsMiddle agedPrognosisYoung adultArticle0003405500000072-s2.0-8490625689177883951-224942235Cell biologyCirculating Microrna; Stomach Neoplasms; Breast NeoplasmsMicroRNABRCA1 proteinBRCA1 protein, humanBRCA2 proteinBRCA2 protein, humanMIRN335 microRNA, humanMirnlet7 microRNA, humanAdultAgedAmino acid substitutionArticleBlood samplingBreast cancerCancer prognosisCancer resistanceCancer susceptibilityCancer tissueControlled studyFamilial cancerFemaleFrameshift mutationGene expressionGene expression profilingGenetic susceptibilityGermline mutationHumanHuman tissueLet 7a geneMajor clinical studyMissense mutationMutational analysisOncogeneOnset ageReal time polymerase chain reactionSequence analysisTumor suppressor geneBreast tumorDown regulationGene expression regulationGeneticsGermline mutationMiddle agedPathologyPrognosisYoung adult