Elshafae, Said M.Dirksen, Wessel P.Breitbach, JustinYuan, ShiyuKantake, NorikoSupsavhad, WachiraphanHassan, Bardes B.Attia, ZayedAlstadt, Lucas B.Rosol, Thomas J.2023-10-262023-10-262020-06-01Elshafae, S. M. vd. (2020). "Canine prostatic cancer cell line (LuMa) with osteoblastic bone metastasis". Prostate, 80(9), 698-714.0270-41371097-0045https://onlinelibrary.wiley.com/doi/10.1002/pros.23983https://doi.org/10.1002/pros.23983http://hdl.handle.net/11452/34573Background Osteoblastic bone metastasis represents the most common complication in men with prostate cancer (PCa). During progression and bone metastasis, PCa cells acquire properties similar to bone cells in a phenomenon called osteomimicry, which promotes their ability to metastasize, proliferate, and survive in the bone microenvironment. The mechanism of osteomimicry resulting in osteoblastic bone metastasis is unclear. Methods We developed and characterized a novel canine prostatic cancer cell line (LuMa) that will be useful to investigate the relationship between osteoblastic bone metastasis and osteomimicry in PCa. The LuMa cell line was established from a primary prostate carcinoma of a 13-year old mixed breed castrated male dog. Cell proliferation and gene expression of LuMa were measured and compared to three other canine prostatic cancer cell lines (Probasco, Ace-1, and Leo) in vitro. The effect of LuMa cells on calvaria and murine preosteoblastic (MC3T3-E1) cells was measured by quantitative reverse-transcription polymerase chain reaction and alkaline phosphatase assay. LuMa cells were transduced with luciferase for monitoring in vivo tumor growth and metastasis using different inoculation routes (subcutaneous, intratibial [IT], and intracardiac [IC]). Xenograft tumors and metastases were evaluated using radiography and histopathology. Results After left ventricular injection, LuMa cells metastasized to bone, brain, and adrenal glands. IT injections induced tumors with intramedullary new bone formation. LuMa cells had the highest messenger RNA levels of osteomimicry genes (RUNX2, RANKL, and Osteopontin [OPN]), CD44, E-cadherin, and MYOF compared to Ace-1, Probasco, and Leo cells. LuMa cells induced growth in calvaria defects and modulated gene expression in MC3T3-E1 cells. Conclusions LuMa is a novel canine PCa cell line with osteomimicry and stemness properties. LuMa cells induced osteoblastic bone formation in vitro and in vivo. LuMa PCa cells will serve as an excellent model for studying the mechanisms of osteomimicry and osteoblastic bone and brain metastasis in prostate cancer.eninfo:eu-repo/semantics/openAccessEndocrinology & metabolismUrology & nephrologyBoneCanineDogMetastasisOsteoblastProstate cancerTumorRich tetratricopeptide repeatOsteoclast differentiationKappa-bExpressionRunx2OsteopontinBreastModelOsteoprotegerinSialoprotein3T3 cellsAnimalsBone neoplasmsCell differentiationCell growth processesCell line, tumorDogsHeterograftsMaleMiceMice, nudeNeoplasm metastasisNeoplastic stem cellsOsteoblastsProstatic neoplasmsTumor cells, culturedArticle0005292941000012-s2.0-8508397017669871480932348616Endocrinology & metabolismUrology & nephrologyCleidocranial dysplasia; Osteoblasts; Transcription factor RUNX2Hermes antigenLuciferaseMessenger RNAOsteoclast differentiation factorOsteopontinTranscription factor RUNX2UvomorulinAce-1 cell lineAdrenal metastasisAlkaline phosphatase assayAnimal cellAnimal experimentAnimal modelAnimal tissueArticleBioassayBiological mimicryBone metastasisBrain metastasisCalvariaCancer growthCanine prostatic cancer cell lineCastrationCd44 geneCell functionCell proliferationControlled studyDogE cadherin geneGene expressionHeart left ventricleHistopathologyIn vitro studyIn vivo studyInoculationLeo cell lineLuma cell lineMaleMc3T3-E1 cell lineMetastasisMonitoringMouseMyof geneNewbornNonhumanOssificationOsteomimicryOsteopontin genePriority journalProbasco cell lineProstate cancerProstate cancer cell lineRadiographyRankl geneReverse transcription polymerase chain reactionRunx2 geneSubcutaneous tissueTibiaTumor xenograft3T3 cell lineAnimalBone tumorCancer stem cellCell differentiationCell growthGeneticsNude mouseOsteoblastPathologyPhysiologyProstate tumorTumor cell cultureTumor cell lineXenograft